Does cell culture medium contain significant activity of catalase?Could it be a component of FBS (does inactivation of serum inactivate catalase too?) Or is it released from necrotic cells like LDH is released ?
Jonathan D Stallings
Serum is known to have catalase activity as the result of several pathological circumstances, clinical and experimental. You can test for this activity with a kit, such as the OxiSelect Catalase Activity Assay Kit, Colorimetric or something similar.
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Samah Jassam
There is catalase in serum, but you can inactivate the catalase activity via heat inactivate the serum before you add it to the media by incubating the serum for 30 min. in 56`c.
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