I performed proteomic analysis on CD and I believe that the protein I worked on could be linked to ATP. Could someone tell me if ATP has optical activity in the range of 200 to 260 nm?
Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.
01 August 2024 10,046 1 View
Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...
27 July 2024 5,748 0 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
Hi, I have performed an Annexin V apoptosis study using flow cytometry and got quite good results. I doubt how to represent the quadrant percentages in the final bar graphs. Some papers only show...
13 July 2024 356 3 View
Hello dear researchers, I would like to know about available in silico tests that could predict the activity of ligands (small molecules) against the target protein. I have performed docking...
08 July 2024 4,724 4 View
seti or search of intelgenece articial nee to be fixed due to masss solar systems outhere thta expelled laser beam light ollimated wel beign so is neede to modificated the seti in a new device of...
21 June 2024 5,059 1 View
Hello everyone! I am currently working with the model organism Ectocarpus (filamentous brown algae). Our team is trying to standardize a protocol for RNA extraction using low biomass (around 10...
18 May 2024 3,862 1 View
TG measurements on the Hitachi STA7300 equipment started to have different results for the same material after some time of use Some tests have already been carried out to try to correct it: Gas...
17 May 2024 7,438 4 View
tener una relacion el ejercicio físico con actividades de equilibrio
16 May 2024 5,290 2 View
Two values will be used for your calculations here: N2 = N1 and N2 = 2N1. Your programme should carry out the following steps: see the attached documents. Thank you in advance
29 April 2024 8,957 0 View
Hello everyone We are building an atomic structure model against a cryo-EM map of AAA+ ATPase. Considering that AAA+ polymerizes when ATP is added, we collected data for AAA+ mixed with ATP. Our...
04 April 2024 4,640 2 View
I am trouble shooting a malachite green ATPase assay using a kit from Sigma (MAK307) for a cytosolic protein that converts ATP --> ADP + Pi and am wondering what cheap + commercially available...
30 March 2024 9,659 1 View
Hello! I'm plannning to investigate the relationship between mitochondrial mass/function and epithelial homeostasis/differentiation. Are there any activators(drug) of mitochondria interms of...
27 March 2024 8,273 0 View
I need to order an ATP Assay Kit for my work, and I found this kit on the BT Lab website. Has anyone tried the kits from this site? Because this is the first time I will order from them.
27 February 2024 6,940 1 View
Hello, I performed the ADP-Glo kinase assay. In that case, samples(active protein+substrate+ATP+Kinase buffer+w/wo test compound mixture) were incubated for 45 minutes in 30℃. In the next...
27 December 2023 8,915 2 View
I am working with HepG2 cells to observe effects of Simvastatin treatment on the cells. We are aiming to identify a dose response associated with levels of ATP, metabolic activity, and CoQ10...
29 November 2023 6,062 0 View
I am currently trying to develop an ATP concentration measurement protocol for my human cultured cells. However, I have found little to no detailed protocol for ATP extraction using TCA...
27 November 2023 7,980 1 View
Hi dear All. I'd like to know simplest protocol to detect ATP binding assay (ATP binding with protein). I read a couple of research about this but I want to know common methods. Thanks for your...
19 November 2023 7,412 0 View
process of human obtaining energy in cellular respiration
28 October 2023 7,303 1 View
I’m having issues with the stability of ATP. My lab has purchased multiple brands of ATP (Sigma # A6419; SantaCruz #SC-202040), each of which seem to come hydrolyzed. My lab uses the Malachite...
18 October 2023 3,648 0 View