In which species you wish to amplify this gene?

Dear Sachinandan,

 I'm working with my colleagues on psoriasis patients. We need to investigate the presence of mutations in this gene.

Hi,

if you are interested in variations (mutations) in that gene you have to do conventional PCR and Sanger sequencing. In the case that you want to search only one specific variation (mutation) you can design real-time PCR with specific probe.

Hi,

Thanks for your answer.

Regards

Do not hesitate to contact me with further questions. Good luck.

Thanks again. I'm appreciated for you.

Regards

Nibras

It's very important question