We are doing an interface to control the MFCs but we are having trouble with the flow values displayed by the 247D power supply. We think we are not using correctly the scaling control factor, does anyone know how to use it for a 100sccm CH4 MFC.
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
14 July 2024 177 4 View
Hi everyone, I am currently writing my masters thesis about Strategic Sponsor Acquisition in Youth Women's Soccer. The aim of the study is to identify potential sponsors for a specific youth...
04 July 2024 9,001 0 View
Hi i want to post this in various groups, how do i do that ? hey, please do my survey when you get the chance, it will take 15 minutes tops x I am looking for UK secondary school teachers to...
30 June 2024 9,594 2 View
We're using LB media, 1mM IPTG, and letting it incubate at 18°C over night. Will adding glucose or something else to the media increase bacterial expression?
06 June 2024 6,071 1 View
Hi I am trying to set up the reactions with my plasmid, insert and RE. Usually the standard final volume is 20 uL or 50 uL (it depends on the protocol provided by the manufacturer) with 1 ug of...
28 May 2024 1,105 3 View
Hi everyone, I'm having trouble with my hydroponic cultures for Arabidopsis thaliana, since lately I've started having algae contamination on them. I have tried using several sterilization...
21 May 2024 4,840 1 View
I'm a doctoral student with early experiences with recombinant protein synthesis. Since I still have to start my experiment and I do not yet know the quantities of DNA and plasmid that I will...
19 May 2024 5,171 3 View
What is recommended as a strong and current reference of qualitative phenomenological analysis ?
16 May 2024 2,142 1 View
I am looking for a some chapter or book related to the statistical analysis of mRNA transcriptomics. Thank you;)
09 May 2024 6,543 2 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View