How to detect phosphorylated protein by western blot ?

Dear expertise, 1) I have some trouble to get reproducible results of phosphorylated protein from western blot. Please give me some ideas and suggestions to get the perfect/reproducible results...

15 March 2020 6,690 4 View

Could anyone help me with TEM analysis of my bacteriophages?

26 January 2015 9,759 17 View

Could anyone suggest me a method for purifying phages particles in the SM buffer for TEM analysis ?

I performed TEM analysis for my bacteriohage.repetitively I got only salts images how to avoid the presence of salts during TEM analysis.

24 December 2014 6,470 6 View

Why do my Vibrio species change their nature of yellow color to green color on TCBS plates after 36 hour incubation?

22 May 2014 6,754 8 View

Why did I get a small amount of plasmid DNA during bacterial Genomics DNA extraction/ isolation?

see above

09 March 2014 2,327 6 View

Can someone help with computer-aided drug-design platform using PyMOL?

How to The file Settings_Linux.txt needs to be downloaded and copied to your home directory? reff site: http://people.pharmacy.purdue.edu/~mlill/software/pymol_plugins/install.shtml I have...

08 January 2014 2,149 3 View

How to extract and purify the Bacteriophage particles against pathogenic bacteria? - please suggest me good ideas and research articles

Need suggestion, good ideas and research articles

17 June 2013 6,974 0 View

Could anyone tell me how to calculate the adsorption rate of bacteria and bacteriophage? with any time duration and/or mathematical calculation

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12 June 2013 1,004 3 View

How to prepare adherence cell line from THP-1 suspension cell line ?

Dear experts, I would like to work on THP-1 as adherent cells without changing its natural. Is there any method or protocol for develop adherence cell from suspension cell lines?

01 January 1970 9,871 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View