I want to know whether leukotriene stimulation to human mast cells can release IL-13. Does anyone know about it?
Does anybody know how many endothelial cells are present in the heart of an adult mouse?
23 July 2023 8,201 1 View
Does anyone know the diameter (size) of mouse heart endothelial cell?
08 July 2023 1,480 1 View
I want to know whether LPS+ATP combination induces inflammasome activation in HUVEC or endothelial cells. Thanks
08 March 2023 5,173 1 View
I'm using a cycloheximide chase assay to look into the stability of a protein. Despite giving the cells cycloheximide (100 uM) over a number of time periods (0, 12h, 24h, and 36h), I was unable to...
19 January 2023 234 0 View
Greetings everyone I am trying to etch some additively manufactured CuCrZr alloys but i am not able to get the melt pool can anyone give me some suggestions I have tried with FeCl3 HCL...
16 January 2023 4,409 4 View
I need to understand the processing route of non-equiatomic high entropy braze foils
18 September 2022 9,198 0 View
I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?
01 March 2021 9,899 1 View
I performed HE staining of intestinal section of wild type (WT) and Knockout mice (KO). I found that the staining intensity is reduced in case of KO mice. There was no difference in villi length...
20 July 2020 1,442 0 View
I am working on a study to understand tourists poor behaviour towards beach cleanliness. For that I am collecting quantitative data. As part of the research, I am also willing to understand the...
15 July 2020 7,494 15 View
I am trying to perform HE staining of small intestine. I used OCT solution to make sections. After HE staining when I look my slides under microscope, I do not get intact villi. In much of the...
15 February 2020 2,456 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View