Does anyone know where to order a generic flow through device for assay development?

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How to analyse quant data with repeated measures?

How can I analyse the following data. Each of 30 people have answered 10 questions (each question has 3 response options). Each person has completed these questions in real time while in specific...

20 June 2024 8,852 4 View

How to do an APA Format Table of a Repeated Measure MANOVA?

I've have been digging on the internet and I could only find one-way ANOVA APA format table example.

15 May 2024 9,390 1 View

SPME: Why is this happening with the SPME fibers?

I have been using a plain gray SUPELCO SPME fiber for direct immersion in 1% KOH, and in one recent occasion, I left samples running (around 10), and I came back to a broken fiber. I tried to...

15 May 2024 6,922 2 View

How do you formulate a literature review?

09 March 2024 4,481 4 View

Five researchers have asked me on your site for copies of some my published articles. How can I do that through your site?

Is the only way people can get copies of articles from an author, is for the person asking to go through the CCC site and pay for the articles at those high rates? Is there anyway I can just send...

28 February 2024 9,490 1 View

I would like to know if I can gain permission to use the modified early warning score system from an article written below for my practicum project?

Predicting Cardiac Arrest and Respiratory Failure Using Feasible Artificial Intelligence with Simple Trajectories of Patient Data. Vol. 8, Iss. 9, (August 29, 2019). DOI:10.3390/jcm8091336Kim,...

24 February 2024 4,638 0 View

How long does it take until I receive the full text version if I have requestet one?

I need an Article for my bachelor-thesis until friday

29 January 2024 8,117 0 View

Global use of facial recognition in boarding pass check?

how it has been used

19 November 2023 1,196 0 View

What STING/TMEM173 unconjugated, monoclonal antibody would people recommend for mice?

Looking for a good STING/TMEM173 unconjugated, monoclonal antibody, reactive in mice for western blots, immunoprecipates, and potentially immunofluorescences. Ideally, I'm looking for antibodies...

18 September 2023 3,142 2 View

How does the Electric Power Distribution System operate?

This query or question will enlighten me further regarding how electrical power distribution networks operate from the power distributor to residential houses.

03 June 2023 1,584 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

How to assess the viability and manage debris in fish primary cell cultures?

Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...

10 July 2024 4,918 2 View

What factors could be contributing on low tumor take in female Nude mice?

Hello everyone, I'm working with a PA-1 ovarian cancer cell line xenograft model in female nude mice. My cells are at 372 passages, the cell repository bank themselves gave at 352th passage....

05 July 2024 5,860 1 View