What STING/TMEM173 unconjugated, monoclonal antibody would people recommend for mice?

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How to analyse quant data with repeated measures?

How can I analyse the following data. Each of 30 people have answered 10 questions (each question has 3 response options). Each person has completed these questions in real time while in specific...

20 June 2024 8,852 4 View

How to do an APA Format Table of a Repeated Measure MANOVA?

I've have been digging on the internet and I could only find one-way ANOVA APA format table example.

15 May 2024 9,390 1 View

SPME: Why is this happening with the SPME fibers?

I have been using a plain gray SUPELCO SPME fiber for direct immersion in 1% KOH, and in one recent occasion, I left samples running (around 10), and I came back to a broken fiber. I tried to...

15 May 2024 6,922 2 View

How do you formulate a literature review?

09 March 2024 4,481 4 View

Five researchers have asked me on your site for copies of some my published articles. How can I do that through your site?

Is the only way people can get copies of articles from an author, is for the person asking to go through the CCC site and pay for the articles at those high rates? Is there anyway I can just send...

28 February 2024 9,490 1 View

I would like to know if I can gain permission to use the modified early warning score system from an article written below for my practicum project?

Predicting Cardiac Arrest and Respiratory Failure Using Feasible Artificial Intelligence with Simple Trajectories of Patient Data. Vol. 8, Iss. 9, (August 29, 2019). DOI:10.3390/jcm8091336Kim,...

24 February 2024 4,638 0 View

How long does it take until I receive the full text version if I have requestet one?

I need an Article for my bachelor-thesis until friday

29 January 2024 8,117 0 View

Global use of facial recognition in boarding pass check?

how it has been used

19 November 2023 1,196 0 View

Could you please help me respond to my dissertation research questionnaire?

Hi everyone. My name is Judith Ejiofor. I am a Master's students at De Montfort University, Leicester. I am doing a research on "The Moderation Role of Percieved Risk: The Relationship between...

19 April 2023 7,878 2 View

How can social media promote food tourism?

Food has become a major tourist attraction in many developed and developing countries, and it is an important part of the visitor experience. However, the marketing potential of social media...

16 March 2023 1,597 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View