Does anyone know where I can synthesize DNA fragments labeled with thiol groups? I want to immobilize DNA on different surfaces.

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What are novel cryptographic protocols that enhance email security?

what are novel cryptographic protocols that enhance email security?

13 June 2024 7,090 2 View

Algae contamination on hydroponic cultures for Arabidopsis thaliana?

Hi everyone, I'm having trouble with my hydroponic cultures for Arabidopsis thaliana, since lately I've started having algae contamination on them. I have tried using several sterilization...

21 May 2024 4,840 1 View

Hello, very good day everyone. I wanted to ask how many rpm to obtain the separation of a drug sample in wastewater with activated sludge?

I need to separate my drug from a sample with activated sludge and residual water

17 April 2024 9,601 2 View

How can I see all points in a 5-95 percentile Boxplot in Graphpad?

Hi! I am trying to do some boxplots in graphpad. I would like to use the 5-95 percentile boxplot option, but if I choose this option I do not know how can I also see the individual values of each...

17 April 2024 6,093 0 View

Where can I get Biological Materials Science Book (Myers and Chen, 2014)?

I know it may be controversial, and I favour crediting the authors. However, knowledge should also be accessible to student researchers. My doctoral colleague and I would like to access the book,...

12 March 2024 1,333 4 View

Dissolved 13C6-L-Leucine in saline stability at -20/80ºC?

I am working with isotopic tracing studies. I was wondering if we can resuspend the whole vial of 13C6-Leucine with saline, aliquot it and freeze it at -20 or -80ºC? Is it stable? For how long?

05 March 2024 2,414 1 View

Do existing EIS models overlook electron magnetic moments, affecting accuracy? How can insights from Faraday's experiments improve this?

Dear ResearchGate community, I recently engaged with a compelling video discussing the Maxwell and Faraday's paradox, accessible via the provided link. Within this content, three experimental...

27 February 2024 964 0 View

How to get B.inp GGA for generate vdW pseudopotential of atom in SIESTA?

On the page I currently cannot download it, or if you can guide me to generate it.

21 February 2024 4,374 0 View

¿What is the affinity of serotonin (endogenous) for the 5-HT1 and 5HT2 receptors?

I need to know if there are measurements or studies on the affinity of serotonin (endogenous) for the 5-HT1 and 5-HT2 receptors

03 February 2024 5,878 3 View

Why MiFish Primers amplify when they're alone but not when they have an overhang sequence (Illumina adapter + tag)?

Good day everyone. I'm working with eDNA Metabarcoding for freshwater fishes in Brazil, I ran tests amplifying positive controls and samples with the primer MiFish-U (Miya et al 2015) alone (no...

11 January 2024 5,109 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

What does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition of \lambda?

For a partition of an integer \lambda, what does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition, , with \lambda_j being the j-th element of \lambda?

23 July 2024 8,008 1 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,075 3 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View