Does anyone know where I can get the amino acid or nucleotide sequence of a high fidelity taq polymerase?

More Benjamin Guerra Contreras's questions See All

What is homomenthyl cinnamate?

the molecule name is citated in old literature about organic sunscreen molecules

10 July 2024 5,576 2 View

Does anyone knows about any protandrous fish inhabiting the Mediterranean Sea, smaller than 20 cm (adult) and that change sex relatively quickly?

Other than Sparids

27 June 2024 1,809 0 View

Is there any evidence that shows how ambient temperature affects insects behaviour?

I'm a student of biological sciences and I need to collect information about how ambient temperature affects insects behaviour to answer the question: " which insects are more active in...

15 June 2024 367 3 View

Very urgent. Where we can get some of these plant species (details see description box) in Nigeria?

1. Vachellia drepanolobium 2. Vachellia horrida 3. Vachellia karroo 4. Vachellia nilotica 5. Vachellia oerfota 6. Senegalia polyacantha ? [email protected] +2348061166247 Thank you.

05 June 2024 4,858 1 View

How can I change the coordinate system in Abaqus using Python?

Hello, I'm writing Python scripts for Abaqus and I'm facing a problem. I need to change the coordinate system in a .odb file before extracting data but I'm stuck. I can create my coordinate system...

30 May 2024 7,431 3 View

How to do an APA Format Table of a Repeated Measure MANOVA?

I've have been digging on the internet and I could only find one-way ANOVA APA format table example.

15 May 2024 9,390 1 View

Can you explain the difference observed between my PFGE and WGS results for E. coli comparison?

Recently i did PFGE and WGS comparison with the same set of E coli strains. In my mind, if PFGE give two different pulsotypes, it is sure that strains are not the same. But when i did WGS, i...

12 May 2024 8,107 2 View

Se puede mejorar la eficiencia de un secador de café a cielo abierto tipo invernadero, desde la arquitectura bioclimática?

El secado del café es un proceso crucial que determina la calidad del producto final. Sin embargo, los secadores tradicionales a cielo abierto tipo invernadero presentan limitaciones que afectan...

09 May 2024 863 0 View

Is there a commercial antibody against insect resilin?

Dear community, I want to perform an analysis of the risilin localization within the legs of myriapods and would like to visualize the localization of the resilin components in different...

07 May 2024 9,160 3 View

How do I specify the argument "time" in the function "create.matrix" (R package fossil)?

I would like to use the "create.matrix" function (R package "fossil") to create a matrix of taxa, localities, time and abundance. In the function, time is specified by the arguments "time.col" (=...

28 April 2024 3,545 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Katie A S Burnette

Those are protected by a patent and they are cheap to buy. Why bother making your own when it's commercially available?

I'd recommend you choose a different project.

Chen Jingwen

Hi Benjamin,

You may make an inquiry at Alfa Chemistry, they offer you advices and kinds of good-quality chemicals.

https://www.alfa-chemistry.com/inquiry