Luciferase activity increase expression but real-time no expression/decrease ?

Hi, has anyone exprience, the luciferase assay resulted in increasing the expression but by the analysis using real-time it is low/no expression at all. Is it because post-transcriptional...

10 November 2017 2,111 4 View

Why luciferase activity decrease when concentration increase?

I was working with luciferase assay to see the ability of my compound as a ligand for receptor. The result somehow does not follow dose dependent manner. it reachs optimum fold change at low...

07 August 2016 3,177 3 View

What is the meaning of fraction size and pool in ion exchange chromatograph?

Hi fellow friends...I'm interested in identify and isolating polyhydroxy alkaloid (imino sugars) from my sample (plant). I've gone through many journal and most of it use ion exchange resins in...

05 June 2016 3,966 3 View

What is the meaning of negative value percentage of inhibition? The value of absorbance of sample are higher than the absorbance of negative control?

I have carried out the MTS test several times. The same dilution factor and sample. The results show that the absorbance of sample are higher than the control. When I plotted the graph percentage...

11 December 2015 1,460 7 View

I want to isolate polyhydroxy alkaloids using IE-X. What is the best column (size, type and etc.) and the best resins should I used? Thank you

Isolate using ion-exchange chromatography.

01 February 2014 5,178 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View