Zuriah Ismail

6 Questions 6 Answers 0 Followers

Questions related from Zuriah Ismail

Luciferase activity increase expression but real-time no expression/decrease ?

Hi, has anyone exprience, the luciferase assay resulted in increasing the expression but by the analysis using real-time it is low/no expression at all. Is it because post-transcriptional...

11 November 2017 2,170 4 View

Why luciferase activity decrease when concentration increase?

I was working with luciferase assay to see the ability of my compound as a ligand for receptor. The result somehow does not follow dose dependent manner. it reachs optimum fold change at low...

08 August 2016 3,265 3 View

What is the meaning of fraction size and pool in ion exchange chromatograph?

Hi fellow friends...I'm interested in identify and isolating polyhydroxy alkaloid (imino sugars) from my sample (plant). I've gone through many journal and most of it use ion exchange resins in...

06 June 2016 4,045 3 View

What is the meaning of negative value percentage of inhibition? The value of absorbance of sample are higher than the absorbance of negative control?

I have carried out the MTS test several times. The same dilution factor and sample. The results show that the absorbance of sample are higher than the control. When I plotted the graph percentage...

12 December 2015 1,560 7 View

Does anyone know where I can buy these resins?

1. Amberlite IR-120B (H+ form) 2. Dowex 1x2 (OH- form) 3. Amberlite CG-50 (NH4+ form)

04 April 2014 1,673 4 View

I want to isolate polyhydroxy alkaloids using IE-X. What is the best column (size, type and etc.) and the best resins should I used? Thank you

Isolate using ion-exchange chromatography.

02 February 2014 5,232 1 View