Huong Nguyen Lan

4 Questions 24 Answers 0 Followers

Questions related from Huong Nguyen Lan

May anyone suggest me, which is the best method use for analyzing refolding protein?

I used Thermo scientific Pierce Protein Refolding Kit for refolding protein. And the gene which I am using has 56kDa and no expressed in soluble protein. How can I ensure that the solubilized...

08 August 2016 3,460 12 View

Is it okay to use my gene even though if differs from the gene bank original by one nucleotide?

For my bacteria gene, after I got a sequence from the company I compared with the original gene from the gene bank. The result was that my gene differed by just one nucleotide, so that changed 1...

04 April 2014 7,775 3 View

May anyone tell me how to make 10x12cm SDS-PAGE with 20% concentration?

My protein is just 13.6 kDa so I want to see that band. who else can help me? Thanks in advance

03 March 2014 7,663 12 View

Can anyone help with expressing my protein?

My gene belongs to Actinomycetaceae family and I want to do heterologous expression that gene in E.coli. I did this work from 4 months ago, but till now, I could not get any positive results. Do...

03 March 2014 4,026 10 View