Is it possible to remove the cells from a tissue and keep only the extracellular matrix for proteomics?

Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....

14 February 2021 1,602 4 View

MHC Rabbit Genome Annotation? Domain annotation of each MHC Rabbit Gene?

I want to find a database(s) or a method where I can find and bulk download all the rabbit MHC genes and domain annotation. In humans using Ensembl, all MHC genes have names and domains but in...

28 January 2021 7,722 2 View

How to interpret the quality level of animal studies by using CAMARADES tool?

We have been working in a systematic review of animal studies and are performing risk of bias and quality assessment using SYRCLE and CAMARADES tools, respectively. Thereby, we would like to...

26 October 2020 7,171 1 View

Has anyone found a way to reliably stain microglia in postmortem frozen brain tissue?

Antibody based approaches aren't working as well as for other cell types, even IBA1 isn't working that well. Does anyone know of a histological/chromogenic stain for microglia? Or any other way?...

13 September 2020 5,927 3 View

Tips on reconstitution of lyophilized material with a small volume?

Has anyone got any advice on how to reconstitute lyophilized material, contained in a 3mL glass vial, through an igloo cap, with only 125uL dilution buffer? I find that the volume is so small that...

21 August 2020 4,347 3 View

Does anyone have a simple and inexpensive protocol for supplementing seawater for seaweed in vitro (laboratory)culture?

Does anyone have a simple and inexpensive protocol for supplementing seawater for seaweed in vitro culture? I am looking to replace the Provasoli medium with something simpler and cheaper (I have...

10 August 2020 302 3 View

What is statistical meaning of testing in machine learning?

Machine learning model, normally, is applied on new data for testing. If the accuracy of the model is below a certain threshold, the model is rejected. But why? This is just a random sample, the...

25 July 2020 8,615 4 View

Why Autodock Vina gives me an error when it is writing the output file?

Good afternoon, I was a performing a docking trough Autodock Vina, following a tutorial from the Scripps. I sent the corresponding commands to run the docking using a configuration file that I...

15 March 2020 2,328 2 View

Could someone help me with my PCR reaction for genotyping?

I used to genotype my KO animals following this protocol: I extracted the DNA from the tail using a Sigma reagent and I performed the PCR reaction with a Promega Green MIX. It worked well, but due...

11 March 2020 7,926 1 View

Is it correct to consider EEMS (Petkova et al 2016) results when observed and fitted dissimilarities are not correlated?

I am using EEMS to analyze a sample of rodents (Calomys venustus) genotyped at microsatellite loci, living in an agricultural landscape. According to diagnostic scatterplots provided by EEMS, data...

04 March 2020 4,411 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View