Has anyone got any advice on how to reconstitute lyophilized material, contained in a 3mL glass vial, through an igloo cap, with only 125uL dilution buffer? I find that the volume is so small that it cannot overcome its own superficial tension and viscosity, so it is really hard for me to rotate the vial and ensure that all the internal surfaces have been reached. In fact, even spending a long time rotating the vial, I have no guarantee that I managed to recover all the powder. Unfortunately changing the vial size or diluent volume is not an option, as it would require full requalification of the manufacturing process. Any technical tips?
Dear all, better to specify the material under investigation to get specific answers. Please check the following documents. My Regards
https://doi.org/10.1016/j.xphs.2020.02.006
https://doi.org/10.1016/j.ejpb.2015.07.029
https://patents.google.com/patent/WO2011082196A2/en
https://www.avivasysbio.com/protocols-procedures/reconstitution-storage-instructions
https://www.randox.com/reconstituting-lyophilised-controls/
https://experiments.springernature.com/articles/10.1007/978-1-4939-8928-7_9
Hi Maimoona Qindeel and Abdelkader BOUAZIZ thank you very much for both your answers.
Unfortunately I cannot provide much more detail, for confidentiality reasons. I can say that the reconstitution is for a lyophilised toxin to be used in a cell bioassay. This is done in a contained environment, using pipettes, as opposed to syringes. Maimoona, could you please send me a link to a paper or protocol that explains how to reconstitute through a sonicator? The concept is totally new to me.
I hope this added detail is useful. Thank you again!
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