Is it possible to remove the cells from a tissue and keep only the extracellular matrix for proteomics?

More Marina Pavlou's questions See All

MHC Rabbit Genome Annotation? Domain annotation of each MHC Rabbit Gene?

I want to find a database(s) or a method where I can find and bulk download all the rabbit MHC genes and domain annotation. In humans using Ensembl, all MHC genes have names and domains but in...

28 January 2021 7,722 2 View

Has anyone found a way to reliably stain microglia in postmortem frozen brain tissue?

Antibody based approaches aren't working as well as for other cell types, even IBA1 isn't working that well. Does anyone know of a histological/chromogenic stain for microglia? Or any other way?...

13 September 2020 5,927 3 View

Tips on reconstitution of lyophilized material with a small volume?

Has anyone got any advice on how to reconstitute lyophilized material, contained in a 3mL glass vial, through an igloo cap, with only 125uL dilution buffer? I find that the volume is so small that...

21 August 2020 4,347 3 View

Does anyone have a simple and inexpensive protocol for supplementing seawater for seaweed in vitro (laboratory)culture?

Does anyone have a simple and inexpensive protocol for supplementing seawater for seaweed in vitro culture? I am looking to replace the Provasoli medium with something simpler and cheaper (I have...

10 August 2020 302 3 View

What is statistical meaning of testing in machine learning?

Machine learning model, normally, is applied on new data for testing. If the accuracy of the model is below a certain threshold, the model is rejected. But why? This is just a random sample, the...

25 July 2020 8,615 4 View

Why Autodock Vina gives me an error when it is writing the output file?

Good afternoon, I was a performing a docking trough Autodock Vina, following a tutorial from the Scripps. I sent the corresponding commands to run the docking using a configuration file that I...

15 March 2020 2,328 2 View

Could someone help me with my PCR reaction for genotyping?

I used to genotype my KO animals following this protocol: I extracted the DNA from the tail using a Sigma reagent and I performed the PCR reaction with a Promega Green MIX. It worked well, but due...

11 March 2020 7,926 1 View

Is it correct to consider EEMS (Petkova et al 2016) results when observed and fitted dissimilarities are not correlated?

I am using EEMS to analyze a sample of rodents (Calomys venustus) genotyped at microsatellite loci, living in an agricultural landscape. According to diagnostic scatterplots provided by EEMS, data...

04 March 2020 4,411 2 View

What can be the best method of estimating the air quality in Piraeus Port in Athens?

I want to conduct this research in the summer months, in order to estimate if high temperature and high air pollution are strongly correlated and if temperature is an estimator of the pollution....

09 February 2020 5,512 7 View

Does anyone know where I can acquire UHMWPE for biological tests?

I want to buy UHMWPE, CrCo alloy and alumina, with particles with different sizes. Does anyone know what company sells these products for biological tests?

23 October 2019 3,052 1 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How to find a correlation between coordinates in a curvilinear coordinate system?

Hello. I have a skewed elliptical coordinate system (I use alternative elliptical coordinates). And I need to determine connections between coordinates in order to fetch a covariance matrix of...

28 February 2021 364 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

How can I generate random samples from Bingham distribution in R? How can I construct those symmetric matrices?

in the function rbingham(n,A) in R software how can I construct that A (symmetric) matrix?

24 February 2021 3,528 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Kelly Kim

Hi Marina,

You might be interested in this article : Article High-Performance Acellular Tissue Scaffold Combined with Hyd...

Sima Lev

You can use centrifugation and filtration (0.22 µm). There are many different to isolate exosomes. Ultracentrifiguation is the most common.

You may look at this paper: "Optimized exosome isolation protocol for cell culture supernatant and human plasma" 10.3402/jev.v4.27031