Does anyone know what these black dots on HEK 293 cells are?

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Homa Nath Sharma

If the cells are growing normally, it should be fine. Otherwise, if infected with mycoplasma or any other microorganisms, cells should have detached already.

Ellen A G Chernoff

This is blebbing (small areas of outpouching) of the cell surface. The black spots are an optical effect.

Shaima Raji Abdul Rahiman Sirajudeen

The cells look healthy. The dark dots are granules that are present in the cytoplasm and is common a feature in HEK 293 cells.