14 Questions 19 Answers 0 Followers
Questions related from Shaima Sirajudeen
What is the formally correct term for mouse E13.5—is it "embryo" or "fetus"? At what stage of the development should the two terms be specifically used?
3 weeks ago 8,124 0 View
I am running MCF10a cell culture-based western blots. I got double bands in 8-16% tris-glycine gels, but this did not happen in 3-8% tris-acetate gels. I was getting clear single bands with the...
28 February 2025 8,780 1 View
The common methods for protein quantification mentioned in a lot of publications are Bradford assay and BCA. Due to some technical issues, I am searching other methods for protein quantification....
16 August 2024 1,246 3 View
I am working on MCF10a cells. I started my culture by thawing a fresh vial of cells purchase in the month of February, 2024. I am culturing them in DMEM/F12 media supplemented with horse serum,...
11 March 2024 8,619 2 View
Is there a method to introduce skin fibroblasts into a cancer mouse model? What are the models that may be used for this process?
15 September 2023 3,217 0 View
Hi I'm planning to use SBB staining for some of my paraffin sections. We have seen unstained areas along the tissue sections in H&E stained sections and also while staining for the mucus...
31 October 2020 1,415 3 View
Hello I'm working on vitamin D studies in mice. I want to keep the mice in vitamin D deficient conditions, so I will feed them vitamin D deficient diet and keep a group completely in dark...
09 December 2018 3,655 10 View
I'm culturing micro algae strains and need to monitor the growth. We are trying to do a comparison of the different methods to obtain cell count. The use of absorbance to determine cell count is...
18 December 2017 3,904 5 View
Hi all I'm currently working with THLE2 cells. It's mentioned in the ATCC product sheet that the cells should be cultured on pre-coated (fibronectin, collagen and BSA) flasks. Will it affect the...
13 March 2017 4,014 3 View
I have to fix the hepatocytes that has been cultured in 3D environment. i.e. matrigel and collagen. We have cultured the cells in 96-well plates. So after sample introduction, we want to fix the...
13 December 2016 840 6 View
I'm preparing the samples for my experiment for TEM. I did the resin embedding step. After adding the pure resin I centrifuged the cells at 1,500 rpm 5 minutes but could't pellet it well. The...
23 February 2016 8,022 3 View
I want to do the TEM for HeLa cells. I need a good protocol for preparation of the samples for TEM. i.e. in the pelleted form as well as on the petridish as a monolayer. Could somebody help?
08 February 2016 8,293 11 View
I want to know how to determine the concentration / amount of nanoparticles that should be used for the experiment
17 January 2016 7,606 10 View
01 January 1970 4,079 3 View
