Shaima Sirajudeen

9 Questions 13 Answers 0 Followers

Questions related from Shaima Sirajudeen

What is the doubling time of MCF10a cells?

I am working on MCF10a cells. I started my culture by thawing a fresh vial of cells purchase in the month of February, 2024. I am culturing them in DMEM/F12 media supplemented with horse serum,...

11 March 2024 8,445 2 View

Introduction of fibroblasts into cancer mouse model?

Is there a method to introduce skin fibroblasts into a cancer mouse model? What are the models that may be used for this process?

15 September 2023 3,162 0 View

Can anybody help me with a protocol for Sudan Black B staining for paraffin sections?

Hi I'm planning to use SBB staining for some of my paraffin sections. We have seen unstained areas along the tissue sections in H&E stained sections and also while staining for the mucus...

31 October 2020 1,329 3 View

What methods can be used to keep mice in dark for vitamin D studies?

Hello I'm working on vitamin D studies in mice. I want to keep the mice in vitamin D deficient conditions, so I will feed them vitamin D deficient diet and keep a group completely in dark...

09 December 2018 3,612 10 View

How to calculate the cell count using a spectrophotometer?

I'm culturing micro algae strains and need to monitor the growth. We are trying to do a comparison of the different methods to obtain cell count. The use of absorbance to determine cell count is...

18 December 2017 3,855 5 View

Is it possible to culture THLE2 cells without pre-coating the culture flasks?

Hi all I'm currently working with THLE2 cells. It's mentioned in the ATCC product sheet that the cells should be cultured on pre-coated (fibronectin, collagen and BSA) flasks. Will it affect the...

13 March 2017 3,966 3 View

Does anybody have a protocol for fixing cells cultured under 3D conditions (Matrigel and collagen)?

I have to fix the hepatocytes that has been cultured in 3D environment. i.e. matrigel and collagen. We have cultured the cells in 96-well plates. So after sample introduction, we want to fix the...

13 December 2016 802 6 View

I embedded the samples for TEM in a day old resin and can't pellet it. Is there a way to save my samples?

I'm preparing the samples for my experiment for TEM. I did the resin embedding step. After adding the pure resin I centrifuged the cells at 1,500 rpm 5 minutes but could't pellet it well. The...

23 February 2016 7,946 3 View

How to determine the concentration of nanoparticles to be used in experiments?

I want to know how to determine the concentration / amount of nanoparticles that should be used for the experiment

17 January 2016 7,548 10 View