I am trying to create a lipid milieu in a test tube. I need to pipette in various lipids in chloroform and then dry off the chloroform.
If anyone knows the standard stock concentration of cholesterol in chloroform that will be helpful.
Thanks.
Hello everyone and I hope I find someone who has done this. Has anyone transfected THP-1 cells in suspension culture? If so, I would love a protocol.
12 August 2023 7,331 0 View
The reason I say simpler organic, is I’m aware if you had an organic that can fluoresce only, or phosphoresce only, you can combine them via some alkyl chain, and for most of the time, the new...
17 July 2023 1,506 9 View
I would appreciate help here in avoiding re-inventing the wheel. Has anyone used AAV to transduce THP-1 in suspension culture? I do not want to do this in activated adherent (macrophage-like)...
12 July 2023 4,565 0 View
I am looking for good and preferably free software that will visualize a set of x, y, and z coordinates. I haven't had much luck with ImageJ or MATLAB. Specifically, I am trying to visualize 3D...
28 June 2023 5,115 7 View
Cannot find this article again after first seeing it on ResearchGate and requesting full text.
11 March 2023 222 1 View
Hi, I am currently in my final year at university and for my dissertation I am investigating the propagation of flexural waves in a 2D structure with varying thickness. Currently, I am finding it...
20 February 2023 6,588 1 View
We have our rat brain lysates stored at -80C and are about to start Westerns. We will need to thaw them for BCA quantification, but then want to have several aliquots of this available in the...
31 January 2023 3,048 2 View
We have a protein of interest that seems involved in membrane blebbing. We wish to inhibit membrane blebbing in some of our experiments without inhibiting ROCK/Rho to myosin light chain kinase...
27 January 2023 3,531 0 View
Is it possible to filter out the salt without filtering the sugar, or filter out the sugar without filtering out the salt?
12 December 2022 5,097 8 View
Hello everyone and thanks for reading his question. I found during bacterial protein production that I had set the centrifuge much higher than intended when pelleting intact bacteria. My...
06 December 2022 6,790 1 View
After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...
29 July 2024 9,753 3 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
I need to do a Schiff base reaction starting from a ketone and 1,10-phenanthroline-5-amino. I've seen that both of these starting materials are not very soluble in methanol and ethanol, which is...
23 June 2024 5,925 3 View
and chloroformate. But it takes 10 days to compete the reaction. How can I shorten the time of the reaction?
14 June 2024 2,924 3 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View
Greetings We are trying to study Cholesteryl esters. We start with MeOH/Chloroform extraction and then remove most of the noise by silica column, but the Cholesteryl esters come out with...
02 June 2024 5,545 0 View
We have been trying to extract RNA from brown algae. We have been following traditional CTAB method as modified by Sim et al., 2013. We get a very thick white jelly interface with the Chloroform...
30 May 2024 356 0 View
I've been working off an RNA extraction protocol using trizol and chloroform for several months that worked beautifully until I moved on to our most important samples. After completing them, I...
23 May 2024 2,475 1 View
Im extracting RNA from tomato leaves by the TRIZOL method but since the chloroform phase separation I can see that the aqueus phase is brownish and the pellet is totally brown. When I evaluate the...
21 May 2024 9,910 1 View
For my project, I need to extract RNA from muscle tissue of EEL. I am trying but the yield is low and Purity not good. The 260:230 ratio is always below 1.50 and 260:280 ratio ranges 1.6-1.86 and...
15 May 2024 3,024 2 View