We have a protein of interest that seems involved in membrane blebbing. We wish to inhibit membrane blebbing in some of our experiments without inhibiting ROCK/Rho to myosin light chain kinase pathway.
Hello everyone and I hope I find someone who has done this. Has anyone transfected THP-1 cells in suspension culture? If so, I would love a protocol.
12 August 2023 7,331 0 View
The reason I say simpler organic, is I’m aware if you had an organic that can fluoresce only, or phosphoresce only, you can combine them via some alkyl chain, and for most of the time, the new...
17 July 2023 1,506 9 View
I would appreciate help here in avoiding re-inventing the wheel. Has anyone used AAV to transduce THP-1 in suspension culture? I do not want to do this in activated adherent (macrophage-like)...
12 July 2023 4,565 0 View
I am looking for good and preferably free software that will visualize a set of x, y, and z coordinates. I haven't had much luck with ImageJ or MATLAB. Specifically, I am trying to visualize 3D...
28 June 2023 5,115 7 View
Cannot find this article again after first seeing it on ResearchGate and requesting full text.
11 March 2023 222 1 View
Hi, I am currently in my final year at university and for my dissertation I am investigating the propagation of flexural waves in a 2D structure with varying thickness. Currently, I am finding it...
20 February 2023 6,588 1 View
We have our rat brain lysates stored at -80C and are about to start Westerns. We will need to thaw them for BCA quantification, but then want to have several aliquots of this available in the...
31 January 2023 3,048 2 View
Is it possible to filter out the salt without filtering the sugar, or filter out the sugar without filtering out the salt?
12 December 2022 5,097 8 View
Hello everyone and thanks for reading his question. I found during bacterial protein production that I had set the centrifuge much higher than intended when pelleting intact bacteria. My...
06 December 2022 6,790 1 View
Hello everybody, I am trying to cross link my protein of interest to see if it homodimerizes when phosphorylated and does not when not phosphorylated. We can stimulate autophosphorylation in...
30 November 2022 4,602 1 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...
06 August 2024 1,752 3 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,748 4 View
Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...
01 August 2024 4,606 7 View
what is the best research evidence for psychological interventions for Bipolar?
01 August 2024 6,023 2 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
We are currently recruiting for two studies on postpartum mental health as part of our work at the Perinatal Mental Health Research Lab at Alliant International University. If you or someone you...
30 July 2024 2,950 0 View
The entropy measured of molecular graphs plays a crucial rule. The network structures in some cases are very lengthy calculations to handle. Some author avoid to construct table where as most...
30 July 2024 3,126 0 View
There are many types of symbolic judgements. For example, when an Israeli pilot flattens the home of a Palestinian family (even if they are forewarned), it is natural for them to experience shock...
28 July 2024 2,610 1 View