02 December 2013 5 9K Report

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More Krishna Prasad's questions See All
What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

How combine yolo with Faster R-CNN?

I want a model that is balanced with accuracy or speed, faster rcnn has high accuracy while yolo have fast speed. i am thinking to combine them to get a hybrid model to achieve both speed and accuracy

02 August 2024 3,104 0 View

How to prepare materials before protein-peptide or protein-protein docking?

Hi all, I am conducting research on screening peptides (20-35 amino acids) with potential for treating dengue fever through protein-peptide docking methods. In the protein database, I found the...

10 July 2024 8,037 1 View

Exact mechanism of anti-cancer agents.?

What is the exact mechanism of synthetic agents showed the cell cycle arrest in G0/G1 and G2/M2 phase in flow cytometry analysis.

28 June 2024 5,950 2 View

About the standard of the FRAP in calculation?

Good morning/day/evening salaam I’d like to ask about the FRAP assay again. I have read some articles which represent the data of frap assay as mg trolox equivalent/L, mg ascorbic acid...

27 June 2024 5,239 0 View

Curcumin encapsulation in Bovine Serum albumin (BSA)?

Hey all what are the ways by which an encapsulated drug inside BSA may escape from its encapsulation. We had synthesised curcumin encapsulated BSA by desolvation method followed by centrifugation...

23 June 2024 8,785 5 View

How does a Man-in-the-Middle (MitM) attack work in the context of Transport Layer Security (TLS), and what specific mechanisms can be employed ?

Context In the realm of cybersecurity, Transport Layer Security (TLS) is widely used to secure communications over a computer network. Despite its robust encryption mechanisms, TLS is still...

19 June 2024 6,595 3 View

How can I implement GAFF-LCFF forcefield in GROMACS?

Dear experts, I am new to molecular dynamics and can run simulations on small Liquid crystal systems using GAFF in GROMCS. I wanted to use the GAFF-LCFF forcefield (Optimization of the GAFF Force...

11 June 2024 4,547 2 View

How to Dilute Stock solution of ferrous sulphate in frap assay?

I want to do Frap assay with ferrous sulphate heptahydrate (FeSO4.7H2O) as the standard. I have calculate the molarity which is 1 M or 1000 microMolar as the stock solution. How do I determine the...

11 June 2024 3,760 2 View

Why is colony PCR not working ?

I've been doing colony PCR for last few days but haven't seen any bands on the gel. I've tried both positive and negative controls, but I'm still not getting any bands. Here's my procedure: I use...

07 June 2024 203 2 View

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How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

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Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

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How to convert g/kg Humic acid dose to kg/ha?

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How to improve peaks separation in HPLC?

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Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

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How can I use DFT-D3 correction ( Siesta version 5.0.1)?

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