The granule mainly consists of EPS, bacteria, protozoa and in some cases fungi. Can anyone recommend the best long term preservation of the aerobic granules if I want to reactivate the granules to observe the EPS activity after a 3 month period. My purpose is primarily to monitor the elemental composition and distribution (physical features) using TEM, SEM, and CLSM analytical technique. Further, maybe along the biological activity analysis.
Dear Syafiq:
You can consult the following papers:
-Advances in aerobic granule formation and granule stability in the course of storage
and reactor operation. http://www.ncbi.nlm.nih.gov/pubmed/20728530
-Reactivation performance of aerobic granules under different storage strategies. http://www.ncbi.nlm.nih.gov/pubmed/22542063
where the possibilities and perspective of using stored granule as inoculums for rapid startup is discussed.
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Nico
I'm glad for the information, by the way this is another paper that I just read that shows a result of microbial activity and FISH quantification after the subsequent reactivation of long-term preserved aerobic granules http://www.ncbi.nlm.nih.gov/pubmed/18442901
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