used TaKaRa Ex Taq (Takara bio inc.). The composition of the PCR mixture is:

TaKaRa Ex Taq （5 U/μl） 0.25 μl

10×Ex Taq Buffer 5 μl

dNTP Mixture （2.5 mM each） 4 μl

Template DNA ＜100 ng

cbbM343F 0.2 μM（final conc.）

cbbM1126R 0.2 μM（final conc.）

ddh2O up to 50 μl

Am received from the Author, Thanks for your Support.

But its doesn't work, any other idea to improve the PCR product.