Umapathi Kanagarajan

2 Questions 5 Answers 0 Followers

Questions related from Umapathi Kanagarajan

For 454 pyrosequencing which is the best primer; 27F - 519R or 28F-519R, and what is the benefit of using the particular primer?

Most of the publications mention 28F, but some papers mention 27F, so which one is best? For the environmental samples. My target is bacterial community findings.

14 April 2014 3,287 3 View

Does anyone know the PCR premix composition and concentration of cbbM343F and cbbM1126R?

I didn't get PCR product using general protocol.

16 March 2014 2,176 3 View