Does anyone know the pCK plasmid and it`s structure ?

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Is there any good online workshop on advanced DEA models?

OR ANY study material with step by step analysis & its interpretation using the software?

01 January 2021 993 1 View

How to convert RGB image of a stable diffusion flame captured from a mobile phone camera to thermal image?

I need the temperature profile (a quantitative dataset) of the flame under study. Is there any image processing tool a available which I can utilize for my study?

18 December 2020 2,707 2 View

Future of cadaveric dissection in anatomy teaching after COVID Pandemic?

Cadaver dissection are the best adopted and accepted teaching tool in anatomy teaching from the accident time till date. Does the dissecting room still have a place in educating our under- and...

02 August 2020 2,948 17 View

Can you please describe the below microstructure?

The below micro structure of Spindle which failed during rolling process hot rolled plates , this is having 0.39%carbon, can anyone please explain the micro structure.

12 May 2020 2,116 1 View

How we can calculate 17O hyperfine coupling constant in gaussian?how we can write the input file?

How we can calculate 17O hyperfine coupling constant in gaussian? how we can write the input file?

16 January 2020 5,197 3 View

Why pearlite shows different colours at 500 magnification?

In eutectoid rail steel , Mostly at 500 magnification it shows dark brown , yellowish or off white appearance, why it appears like that because in mild steel it appears in black color

12 December 2019 8,467 1 View

How do I quantify protein extracted from cereal flour with 70% ethanol?

How do I quantify protein extracted from cereal flour with 70% ethanol using Lowry assay ? Standards from protein BSA can not be prepared in ethanol because of low solubility. So which is the...

07 November 2019 4,032 1 View

No title

I have road traffic accident data of last 5 years with different variables. But i don't know type of variables require to fit an econometric model and the name of different econometric models.

18 October 2019 3,713 2 View

Can I use glass cuvettes in FTNIR spectroscopy?

I'm using a PE FTNIR spectroscope and working in a wave length 759nm to 2500nm. I'm broke with quartz cuvette. Can I use a glass container for scanning my material?

14 October 2019 1,715 1 View

How we can calculate the pH effect in gaussian?

04 October 2019 4,819 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View