Does anyone know the pCK plasmid and it`s structure ?

More Niharika Gaur's questions See All

How can I make the CST simulation run faster?

I am trying to simulate a metasurface of 24x24 elements, but even with a GPU of 48GB it takes very long to simulate. I want to obtain the field along the defined curve.

10 June 2024 3,577 7 View

How to inerpret and assign NMR peaks?

I have done NMR analysis of exopolysaccharide samples. I have to identify the monosaccharide composition and the functional groups the NMR peaks corresponds to. Since, my EPS is unknown i am...

15 May 2024 7,631 1 View

Why is my protein concentration increasing after storage at -20°C?

I have a few proteins that I produce recombinantly in E. coli cells. All of them are disordered proteins and are stored in the presence of 4mM DTT at -20°C before use. One of them also contains...

28 November 2023 2,682 2 View

Why do A431 cells form clumps in propidium iodide staining solution?

I've been trying to study cell cycle using PI (propidium iodide + Triton-X + sodium citrate)b staining solution. I trypsinize my cells (A431: skin carcinoma), fix them using 100% methanol and...

20 November 2023 2,443 3 View

How would you describe that robots are not what anthropomorphisation is considered to a young child who sees a robot like a human only?

A young child would describe a robot as a human kind, who does lots of mechanical work, uses its brain, talks back etc. How will that child understand the difference between a robot and...

13 September 2023 211 1 View

Not getting any protein expression. How to tackle the issue?

I am trying to express a novel GlcNAcT enzyme in BL21(DE3). I have successfully cloned it in pET28a vector and confirmed it through sequencing. I tried following previous protocols for GlcNAcT...

19 July 2023 6,064 2 View

Is there any public database that has both chest x-rays and CT Chest available?

The NIH has separate databases for chest x-rays and CT Chest but I am looking for any public one that has both available to compare some variables between both.

12 July 2023 3,530 2 View

Can we use PRISMA if we are using a single database?

Because in PRISMA, there is duplicate removal option. With single source, there is no chance for

31 March 2023 8,609 1 View

How to obtain sensitivity from cst?

I am working on obtaining sensitivity of microstrip patch in cst but I cannot seem to understand how to obtain the plots in the paper ( page 6044/fig 8). I manually derived the formula for...

27 February 2023 5,663 0 View

How to conduct qualitative detection of sialic acid using resorcinol method on TLC plates made with silica gel??

I am qualitatively trying to determine the sialic acid using resorcinol on TLC plates. Can someone help me with the process?

26 January 2023 7,825 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View