Does anyone know the molecular weight of the anti-atox1 antibody?

More Antonio Rosato's questions See All

Can you help me with pharmacopoeia procedure 3.2.1 on arsenic determination?

Hi to all I am writing you in order to get more information about the procedure 3.2.1 on Arsenic determination in glass containers for pharmaceutical use. In the monograph, at the paragraph...

05 June 2018 1,377 3 View

How do you overcome the following issues with S and Os ICP-OES?

hi to all, i'm experiencing several issues with detection of elements such as sulfur and osmium at icp-oes. in particular, i work with an Agilent ICP, and at each analysis, i have to analyse these...

08 September 2017 5,505 3 View

Is it possible to infere from isoelectric point the superficial pH?

hi to all, i would like to estimate the superficial pH on spherical particles having dimension of nanometers. since the surface is regular and omogeneusly coated, i was wandering if could i infere...

02 March 2016 9,046 2 View

Can amicon ultra devices be used at 4°C?

hi to all, I need to use amicon ultra filtration devices at low Temperatures in order to purify a protein sensitive to this parameter. can i use amicon devices in a centrifugue refrigerated at 4°C?

02 March 2016 8,307 4 View

How can I stop protein induction?

hi to all, i was wandering if it is enough to pellet cells (e.coli) from the protein induction medium containing iptg and resuspend them in a new medium without inductor to stop the protein...

01 February 2016 3,643 3 View

Is it possible to co-trasform E.Coli bl21 de3 gold with two iptg inducible plasmids?

hi to all, i was wandering if it is possible to transform bl21 de3 cells with two plasmids. the aim is to espress 2 proteins at once. the two plasmids are both iptg inducible, and ampicillin...

01 February 2016 3,119 8 View

Is it strictly necessary the chloramphenicol in LB agar plates for pLysS cells, or the ampicillin is enough?

If I add chloramphenicol only in the overnight liquid culture, is ok? should the chloramphenicol be present also during the induction of the protein? thank you

31 December 2015 1,255 3 View

Could the OD_600 of a bacterial culture be a clue of protein expression?

hi to all, i have noticed that if i incubate two parallel bacterial cultures in the same conditions ( one where i add IPTG for protein induction and another in which I don't add anything) the OD...

31 December 2015 1,080 7 View

Can glucose polymers be used by E.Coli as carbon Source?

Hi to all, I'm wondering if glucose polymers such cyclodextrins, glycogen and more complex polymers, are metabolized by E.Coli. Thank you

09 October 2015 2,798 4 View

How can I dock a 4Fe-4S cluster into a protein?

hi to all, I'm trying to dock a 4Fe-4S cluster into a protein using the Haddock web-tool. I suppose that the protein I'm using coordinates the cluster by means of specific residues, that I have...

05 June 2015 5,973 0 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

Roberta Galletti

I've never used this particular Ab before, but I'd say that being an immunoglobulin (whichever class) it sticks to the rule 25KDa for the light chains, 50KDa for the Heavy chains = roughly 150 KDa for each monomer (class M Ig usually form oligomers)

Mohamadreza Mirzababaei

it depend to where you buy that

for example in novusbio Molecular Weight is 6 kDa