i need to know the LC50 of Monocrotophos on zebrafish.
I would like to do an insilico study in a medicated milk. So planning to do LCMS for identifying the phytoconstituents of the same. But the experts suggested to extract the protein and fat portion...
09 June 2024 1,281 2 View
I had done calculating MTT assay using Slope intercept method - Excel Four parameter logistic regression model-web portal Using Graphpad prism. Among them which one is the best and suitable for...
01 June 2024 2,576 3 View
Hi everyone, the x=axis is samples sample1= wt sample2= yk∆ sample3=swe∆ So, what is the right code for this symbol ∆? Thank you
16 February 2024 6,116 0 View
Greetings everyone. I have a question regarding protein-polysaccharide complexes from plants. Recently, I heated plants extractions in NaOH and NaCl. During heating process, the colour turns from...
09 January 2024 3,197 2 View
I am doing my Masters Thesis in climate change studies and found an interesting trend of temporal monsoon shifts (happening earlier), less number of rainy days in monsoon and also shift of max...
19 November 2023 4,218 0 View
I would like to identify the phytochemicals from a medicated milk. Confused by the analytical technique for identification.GCMS/LCMS, what will be ideal one.please suggest
19 September 2023 2,235 2 View
I’m currently in the process of selecting a research topic for my doctoral studies. I have a keen interest in the field of acoustic metamaterials. I would really appreciate if you are helping me...
29 August 2023 694 1 View
Planning to do Graduate thesis in the field of DSSAT, Rice crop. Have moderate level data availability. Please suggest me on this. Thank you in advance.
21 April 2023 4,149 5 View
how to transform my results in the EuroQOL EQ-5D + EQ VAS to get an overall score that can be presented as “good, moderate, poor“ quality of life?...
24 February 2023 6,630 3 View
Chloroform methanol should be separated into 2 phases
03 January 2023 8,940 1 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
zebrafish and mouse
19 July 2024 9,158 1 View
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View
I am encountering an issue with my PCR band size. The expected band size is around 450 bp, but I am consistently obtaining bands near 2,000 bp. There are no bands in the negative control, so...
30 June 2024 295 0 View
Hi all, I am looking for advice on what could be going wrong with my zebrafish caudal fin tissue fixation that would cause the sample to fall out of the embedding while cryosectioning. During cryo...
25 June 2024 4,602 0 View
Feeding zebrafish larvae properly is a challenge if we need to grow them. According to standard guidelines, how many grams or milligrams of Artemia should be given to 6 dpf larvae?
13 June 2024 389 0 View
I want to teach a class using zebrafish embryos. It's in the afternoon, so I want to get my hands on some fake, reusable embryos that students can use for practice. Does anyone know of any?
10 June 2024 3,956 1 View
I am using the hanging drop method to develop individual differentiated adipocyte spheroids from the stromal vascular fraction of adipose tissue. With our current timeline they end up 100-300um....
27 May 2024 1,303 1 View
can any budy explain how i calculate the DNA concentration for the 50ul reaction?
22 May 2024 9,557 1 View