Trichoderma virens arg2 gene
Thanks, I tried it, but no results came out for T. virens...
Thank you very much!!! I found it at EMBLA-ENA :)
How can we prevent small peptides (1000 Da) from eluting out of the gel during staining in a peptide gel (tricine-SDS-PAGE, Shagger)? There are different types of fixing solutions, but I'm not...
28 April 2024 1,522 0 View
I have some environmental isolates that we are looking to identify. Morphologically, the majority of the isolates are Bacillus spp. We would like to use the API 50 CHB/E system and would like to...
28 April 2024 9,559 0 View
Hello everybody, I've been trying to fix the FTIR attached to this enquiry but I havent been lucky at all. I would like to know if anyone would be so kind as to help me fix this spectrum or tell...
25 March 2024 7,648 0 View
Medical laboratory technologists are likely to be faced with the challenges brought on by Artificial Intelligence (AI) as laboratory medicine continues to undergo digitization and automation. The...
02 September 2023 6,162 3 View
Artificial intelligence may provide a lot of advance in the modern age, however, there is still some downside that AI has and lacks.
02 September 2023 1,126 2 View
I recently started reading about bioelectrical impedance vector analysis, and I downloaded the BIVA-tolerance excel template (http://www.renalgate.it/formule_calcolatori/BIVAguide.pdf)....
04 October 2022 3,853 2 View
Dear community, I am a long-term Malvern Mastersizer 2000 user (and lover) but now, I find myself using a Sympatec Helos for LD measurements. I would like to know if any user knows or have...
30 September 2021 8,638 0 View
I don't have a microplate reader in my lab now and using a cuvette-type UV-Vis spectro is kinda "wasteful" in terms of how much of the treatment solutions I'll be using. So I thought of using a...
02 May 2021 9,568 3 View
I used 2X Phusion Master Mix during my PCR reaction and after that I run the PCR product (10ul). I can observe a faint band but I do not why it looks like degradation. I attached a picture to ask...
18 March 2021 5,070 4 View
Plant growth and development are regulated by the environmental conditions and availability of mineral nutrients. The uptake of mineral elements is regulated from soil properties and rhizosphere...
03 August 2020 6,708 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
