Dear all, as luck would have it, I reached for my PureLink PCR Micro kit and the wash buffer totally evaporated. Does anyone know the composition of the wash buffer in the PureLink PCR Micro kit? I assume that it's mostly ethanol, maybe 90% ethanol?
Prakash Bhuyar
Before beginning, prepare the Wash Buffer (W1) with 100% ethanol as follows: For K310050 (50 preps):
1. Add 32 ml 100% ethanol to the Wash Buffer.
2. Check the box on the Wash Buffer label to indicate that ethanol was added.
3. Store the Wash Buffer with ethanol at room temperature.
For K310250 (250 preps):
1. Add 160 ml 100% ethanol to the Wash Buffer.
2. Check the box on the Wash Buffer label to indicate that ethanol was added.
3. Store the Wash Buffer with ethanol at room temperature.
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