I've heard people saying they got good results with Adeno associated virus

Dear Monika,

One of the best method for genetically modifying cardiomyocytes is the Magnetofection method. In brief, biomolecule-to-deliver is combined to magnetic nanoparticles via non-specific interaction (electrostatic and hydorphobic). After 20 min incubation, the magnetized vectors are added onto the cells, and cells are placed onto a magnet. The magnetic field then attracts the complexes onto the cell surface. for more inforamtion on this method, you can refer to the link attached to this post.

Some authors have demonstrated the use of Magnetofection to transfect cardiomyocytes, with adenoviruses using AdenoMag (Campbell KF., J Physiol. 2012 - http://www.ncbi.nlm.nih.gov/pubmed/23090949), or with DNA using NeuroMag (Subramanian M., PLoS One. 2013 - http://www.ncbi.nlm.nih.gov/pubmed/23936108), and PolyMag (Bittel DC., Cells 2014 - http://www.mdpi.com/2073-4409/3/3/713).

Magnetofection is generally the method of choice for primary and hard-to-transfect cells.

If you need any supplemental information, or if you would like to try this method, please do not hesitate to contact me at: [email protected]

Good luck with your experiments,

Cedric

http://www.ozbiosciences.com/content/13-magnetofection

Dear Cedric,

I never heard of this kind of transfection method and it sounds interesting. But what I care about is the transfection efficiency. Have you compared with other transfection methods?Do you have some data to show me for reference?

Thank you!

Youhong

Dear Youhong,

sorry for the delay,

please find 4 references for Magnetofection induced transfection or transduction of primary cardiomyocytes (for your convinience I've added direct link to the publications):

DNA: PolyMag:   Bittel DC., Cells 2014, 3(3), 713-723

Adenovirus : AdenoMag: Campbell KF., J Physiol. 2012 Dec 15;590(Pt 24):6363-79.

DNA: CombiMag: Eder P., Cardiovasc Res. 2007 Jan 1;73(1):111-9.

DNA: NeuroMag: Subramanian M., PLoS One. 2013 Jul 31;8(7):e69812.

The magnetofection method is totally non-toxic and can also be used in vivo to target specific area in animals.

If you would like to try this method, please do not hesitate to contact me directly at: [email protected]

best regards,

Cedric

http://www.mdpi.com/2073-4409/3/3/713

http://www.ncbi.nlm.nih.gov/pubmed/23090949

http://www.ncbi.nlm.nih.gov/pubmed/17129578

http://www.ncbi.nlm.nih.gov/pubmed/23936108

Hi Cedric, 

Thanks for your kind help. I will contact you if we need it.

Regards,

Youhong