Does anyone know the best online tool to perform Gene Ontology (GO) enrichment analysis uploading protein sequence?

More Akram Salah's questions See All

How to calculate conductivity using Electrochemical Impedance Spectroscopy's Nyquist plot?

Hello everyone, I am new to this characterization but watching videos on youtube I have already plotted a Nyquist plot (Z' and -Z'') and already fitted models on my material using Zsim. But now I...

29 July 2024 1,376 0 View

MAGeCK Plotting Error: Logarithmic Axis Must Have Positive Limits?

I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...

28 July 2024 9,811 1 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View

What is the angular difference between two sets of Euler Angles?

Let's say I rotate a hexagonal crystal using Euler angles (φ1, φ2, φ3); and rotate another similar crystal using Euler angles (ψ1, ψ2, ψ3). What would be the angular difference between the final...

17 July 2024 5,582 1 View

Getting small dark current when simulating the Photovoltaic device Using the SCAPS-1D?

When I simulate the solar cell or self-power photodetector in SCAPS-1D, I optimize all parameters, Once I check the dark current at 0 voltage, the current is high in a range of (e-16-e-25), as...

06 July 2024 1,782 3 View

How to calculate Capacity retention for Lithium metal battery?

I have prepared half cell. Lithium is considered as anode in the system. Whereas, I would like to know what is actually capacity retention? How it can be calculated mathematically? On what factors...

02 June 2024 4,135 0 View

What is the breakpoint diameter for florfenicol and oxytetracycline in the disk diffusion?

What is the breakpoint diameter for florfenicol and oxytetracycline in the disk diffusion method, indicating susceptibility or resistance to these antibiotics in the tested salmonella strain?

25 April 2024 5,168 1 View

Is there a free trial of the SILVACO TCAD ?

I wan to simulate the 2D Photodetector

22 April 2024 8,756 0 View

Is there a company known that can design an electrode shadow mask for photodetector ?

I want to design electrode shadow masks for photodetectors .The shadow mask can be made from uncontaminated materials. We do not have lithography fabrication at my university, so i want to design...

12 April 2024 2,312 3 View

What is the reason that a Li-ion coin cell is not showing efficiency on neware battery testing system?

I have been working on electrospun membrane for Lithium Ion coin cells. I have tried to control different parameters, such as, thickness, electrolyte amount and many other parameters. I am facing...

26 March 2024 2,786 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View