Does anyone know the best method to measure receptor recycling or trafficking?

More Mitzi Paola Santoyo-Sánchez's questions See All

Could someone please provide a list of journals that accept the application of methodologies in nature-based solutions?

I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.

23 July 2024 6,444 2 View

About mineral exploration?

Are there any techniques at ore exploration that could replace the compass-tape measure of mineralized structures?

14 June 2024 3,254 2 View

What is the minimum amount of liquid and beads I can use for bead-beating in a 2ml vial?

I am planning to extract DNA with QIAamp Mini DNA extraction kit (Qiagen) from DNA Shield microbiome preserved samples. My samples contain small amount of cell, so my goal is to reduce the loss of...

14 May 2024 2,291 2 View

Can I replace some of the buffers from the first steps of QIAamp Mini kit by DNAshield?

I am planning to extract DNA with QIAamp Mini DNA extraction kit (Qiagen) from DNA Shield microbiome preserved samples. My samples contain small amount of cell, so my goal is to reduce the loss of...

14 May 2024 7,178 3 View

How do I measure the virulence intensity of a fungus strain in a host plant?

I need to determinate how virulent is a fungus/oomycete strain-specie in comparative with others. I have plants group with and without fungus symptoms. However I found the fungus sources in both...

06 May 2024 8,974 5 View

¿How can I publish a book chapter without payment?

I almost finished a book chapter about my resent research (modeling of solid biomass combustion using OpenFOAM) and I want to publish it. In the case of a paper the procedure consists of sending...

26 February 2024 2,352 6 View

Application of pulsed light?

What are applications of pulsed light on produce?

19 February 2024 7,686 0 View

Ranges of psychiatric evaluation scales?

What are the ranges of the scales: Social Functioning Scale (SFS): to measure social functioning BPRS (18 items) and BPRS-E (extended version, 24 items): schizophrenia symptoms PANSS:...

27 January 2024 2,960 3 View

I am trying to detect a secreted protein by Western blot, Does Any of you have experimented some problems identifying secreted proteins by WB?

I am trying to detect a secreted protein by Western blot in plan tissue. To this aim, we are using a simple protein extraction protocol based on TX-100 and DTT. Then, I am loading the samples in a...

14 January 2024 1,647 4 View

Best way to fit mosquito incidence model with future climate data?

I have tried with no avail to make a logistic regression and random forest model work. First i fitted a model with climatic measurements as independent variables (2015-2020, almost five years)...

10 January 2024 9,738 1 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

Suggestions for the Annexin V Positive Control ?

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29 July 2024 8,624 1 View

How do we pick data for determination of Validation Acceptance Criteria?

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23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

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17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

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15 July 2024 6,668 0 View

Neil A Bradbury

Bivalent antibodies can cause capping and induce internalization. Also you need to be sure your antibody recognizes an extracellular epitope. Biotinylation is the best way to follow this. We have published a protocol you might find useful.:

Methods Mol Med. 2002;70:323-40.

Analysis of CFTR endocytosis by cell surface biotinylation.

Weixel K, Bradbury NA.

PMID: 11917535

Mitzi Paola Santoyo-Sánchez

Thank you! Neil; I'm going to review

Ashik Jawahar Deen

Hi, I don't know if its going to help but I wanted to share my ideas. You can use TIRF microscopy, combined with special fluorescent tags like IRAP-pHluorin, Dendra2 etc., to visualize recycling of cell surface proteins. You have to choose the best option for your protein of interest. Please look at the following papers for details. Data analysis is complicated but you can adopt the most suitable method for you!

Than you Ashik, in this moment, i have a problem with my cells. But i'm going to review these papers