Which variables can be used to increase/decrease the grain size of czochralski-grown polycrystalline silicon?

Which variables (such as pull speed, melt temperature, annealing temperature/duration, cooling rate, etc.) affect the grain size of czochralski-grown polycrystalline silicon? In what direction...

10 January 2021 2,204 6 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Do you have any application suggestion for automated data collection for surveys?

I'm planning to do survey for about more than 700 people online. Is there has any software you can suggest to collect the data? Thanks

05 October 2020 8,079 7 View

Where can I obtain Streptomyces lividans strains for enzyme production?

I am having trouble figuring out where to obtain Streptomyces lividans strains that are commonly used for enzyme production. Specifically I am searching for the following strains: S. lividans...

25 May 2020 6,205 3 View

What devices are available for percutaneous pH monitoring?

I'm interested in percutaneously measuring local changes in tissue pH in vivo with and without a therapy. What devices are available for percutaneous pH monitoring in large animals?

20 March 2020 2,731 3 View

How to genotype between wild-type and heterozygous mutant zebrafish with only 1 bp difference?

22 January 2020 7,703 4 View

What is the best way to approach building a custom polypeptide?

I am trying to build a custom poly peptide that is ~50 amino acids in length, and it will ideally have a charge of ~+4 at pH 7. I can get the positively charged attributes from incorporating a...

08 December 2019 3,597 1 View

Whole Blood Over Recovering Compared to Blood Plasma?

My Immunoassay is giving higher analyte concentrations for the same sample before plasma separation vs after plasma separation. For instance, I run my assay with whole blood, and I get a reading,...

10 July 2019 7,391 3 View

Why I got full length DNA with Taq but partial length with Pfu ?

I want to over lap two DNA sequence A : 378 bps B : 374 bps Taq PCR protocol : A sequence template 1ul (600pg/ul) B sequence template 1ul (600pg/ul) Foward primer 1ul...

30 June 2019 7,127 4 View

How can I perform a meta-analysis of incidence rates (X events per 100,000 person years) with 95% CI without a comparison group?

I'm performing a meta-analysis, attempting to produce a summary statistic for incidence rate of events of sudden death per 100,000 person years with 95% CI, and eventual subgroup analysis. I am...

11 June 2019 5,867 5 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to get better phase shift in C-V curves for ferroelectric devices?

I tried to sweep my ferroelectric devices from -2V to 2V and then from 2V to -2V and The two hysteresis peaks are off by several microFarads. Should i increase bias voltage? I am doing these...

16 February 2021 8,514 2 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

What are the possible effects of COVID -19 Vaccine/s on the persons already recovered from the disease?

Persons recovered from COVID -19 are having antibodies against the virus inside their bodies. They are having T cells already sensitized to the antigens of the virus. If they are vaccinated (may...

12 February 2021 4,106 22 View