Asraf Hussain Hashmi

8 Questions 13 Answers 0 Followers

Questions related from Asraf Hussain Hashmi

How can I fix virus for electron microscope?

Is there any paper or protocol to fix envelop viruses for EM? Can we fix envelop virus with formaldehyde or formalin?

01 November 2015 2,811 12 View

Can anyone suggest a protocol to prepare sample of mice hepatocytes for Confocal Microscopy?

after fixation of slide with 4% formalin, how to stain them to see anti-protease proteins. i.e. Antibody of respective enzyme and secondary anti-body labelled with FITC?

03 September 2015 2,312 2 View

Why is IFN alpha without pegylation called Standard/Conventional interferon?

IFN alpha 2a or 2b without pegylation is called SIT. Why? any other differences in addition to pegylation?

28 December 2014 7,013 3 View

What is the exact size of IFNa, 2a and 2b? Is there a Database available with this kind of information?

IFNa has 13 genes. Is this classification based on genes 2a, 2b?

28 December 2014 242 1 View

Does HCV infect platelets?

Does thrombocytopenia occur due to HCV infection of platelets like HCV infection of hepatocytes?

21 April 2014 7,640 16 View

Why does Egypt have 15-20% prevalence of HCV?

Egypt has the highest prevalence of HCV. What is the major risk factor or history behind it?

18 April 2014 3,170 26 View

Can metabolites play a very important role in HCV spontaneous clearance?

A lot of research is being done on to investigate the host & viral factors behind HCV spontaneous clearance i.e cytokines, T-cells, Il28B, Kupffer cells etc. Cellular environment is very...

13 January 2014 6,787 11 View

How to isolate RNA of HCV from Huh7.5 cell lines infected with HCV serum?

We use Qiagen RNA extraction kit routinely but when cell lines infected with HCV serum and RNA was isolated by same method there were no promising results.

02 January 2014 4,108 4 View