PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Thin cut brain tissue flaking off of slides?

Hello! I have just encountered a new issue with some tissue flaking off of my slides and wondered if anyone else has experienced this or has any suggestions? The sections were cut at 18 microns...

09 December 2020 4,954 2 View

Sample size in G mellonella studies?

Hi everybody, I am working with G mellonella, and I know that one of the advantages of it is the lack of ethics commit and the 3R principles. However, I am very insecure about the sample size I...

29 October 2020 2,079 1 View

There are any component of gram + bacteria cell wall that can be used as a positive control?

Hello, I am going to evaluate the immunomodulatory effect of a gram positive probiotic bacteria in vitro and I need to use a positive control. I was thinking about LPS, but it's a component of...

11 March 2020 7,369 3 View

Any software for decomposing unitary matrices into quantum circuits?

I have a 32 by 32 unitary matrix representing a doubly controlled quantum operation and need to translate it into a quantum circuit. Are there any applications, preferably free, that can decompose...

05 January 2020 434 3 View

Where can I find a validated questionnaire that evaluates the professional profile and trajectory of graduates of a residency /specialization program?

I am looking for a validated instrument that allows me to collect information about the exit profile of a residency program and his professional trajectory after the program.

04 June 2019 7,709 3 View

Are the results in the PhET Stern-Gerlach simulator correct?

In a course on quantum mechanics I took we were told that for the setting in which the gun shoots a particle with spin orientation +z and the analyzer is perpendicular to this orientation, 50% of...

10 February 2019 416 6 View

Protocol: Conventional PCR HPRT gene?

Hi! I would like to know if anyone has the protocol for HPRT (housekeeping gene) conventional PCR (cyles, temperature...)? I've been searching on internet but I just found for real-time PCR. Can...

22 October 2018 7,984 2 View

Rusle LS parameter formula in ArcGis - Which is the theorical formula behind it?

02 September 2018 3,565 12 View

Are Anfinsen and Levinthal still considered valid in protein folding?

What are the recent developments regarding Anfinsen's idea that the way proteins fold depends on the sequence of amino acids alone? Are there any new insights on Levinthal's treating the native...

19 August 2018 9,320 16 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View