Does anyone know phenotype markers for M2 macrophages in the rat? There are many antibodies available specifically for mouse (FIZZ, Ym 1/2) but not for rat macrophages (or, at least , I am not aware that there are any of these specific for rats).
Does anyone know specific pan macrophage marker in rat? I guess that F4/80 is not a good choice for rats??
I ran a logistic regression model with PTSD, MDD, Nativity, and (PTSD*Born outside the US) interaction term predicting Nicotine Dependence (yes/no). The main effect of Born Outside the US (ref:...
22 February 2022 1,196 2 View
Hello, I need help with choosing a statistical method. I have two groups, each was tested by using a test that is compiled of three subtests. First, I need to estimate the effect of subtest1 on...
05 November 2021 3,996 5 View
I extract DNA from various sludge samples according to the method by Griffiths et.al (2000). By now I did not have any problem, even in case of sludges which contain a great amount of silage and...
14 November 2019 2,619 3 View
Starch concentration range: 10-100 g/L; The additive should not influence the pH value significantly, should be stable at high temperatures (during autoclaving) or suitable for sterile...
29 September 2019 3,154 4 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...
29 July 2024 236 2 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View