I am trying to isolate high quality RNA from marine worms larvae using Direct-Zol RNA microprep from Zymo and the yield is very low. My biggest concern is the homogenization of the larvae once they are in TRIzol.
We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...
02 March 2021 6,036 1 View
Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...
07 February 2021 1,671 2 View
Hello everyone. I am looking for a simple formula to compute the distance in Km between two points inside the Earth. I do not mean the usual problem between to cities (distance along the surface)...
02 February 2021 3,455 4 View
We keep getting this error on our Agilent 6470 TQ. We were told it could either be our N2 generator or filter traps. We've called the manufacturer of our generator and they believe it's working...
21 January 2021 2,969 1 View
Hello I'm using image registration over the bands of the micasense red-edge camera. Using RedEdge band as the fixed image. After removing inliers I can notice that inliers of the NIR band are...
13 January 2021 3,980 2 View
Ethics and moral values have always being an essential part of teaching and scientific research. How do you teach and evaluate these essential subjects in your daily interaction with students?
23 December 2020 5,058 7 View
Hi, does anyone know why TRAP staining faids in FFPE rat femur sections? My protocol: After deparaffination and rehydration i put the slides in Acetat Buffer for 20min, then they go to the...
14 December 2020 3,112 4 View
Anyone with experience in growing JAWS II cells could give me an idea of its doubling time? I intend to use these cells for transfection protocols. Any tips to help with growing and transfecting...
01 December 2020 855 3 View
I have 68nm spherical Au NPs, which gave me a concentration of 1.22 x 10^9 particles/mL. How can I calculate the molarity without the need for TEM?
22 November 2020 8,850 3 View
Hi, I'm trying to run MrBayes 3.2.7 with a matrix of 37 species and 20561 nucleotides. I consistently receive the following error: MrBayes > Execute...
18 November 2020 3,867 3 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...
24 February 2021 8,618 3 View
I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...
24 February 2021 3,623 3 View
I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...
23 February 2021 8,969 1 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
23 February 2021 8,850 2 View
Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...
22 February 2021 5,341 5 View