Does anyone know of any suitable methods for S-adenosylmethionine (SAM) quantification in fibroblasts other than by HPLC-MSMS?

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Ahmad Mohamad Zaiter

Instead of using HPLC MS/MS u can use RP-HPLC ........................it is easier compared to the last.

Imre Molnár

I agree with Ahmed. Use 210 nm UV-wavelength and start from 5% to 90% AcN in 15min, Column: a good C18, f.e. Acquity BEH C18 50x2.1mm, 1.7µm (Waters)

Flow: 0.5 mL/min, Temperature: 30°C, make sure to repeat the gradient runs at least 2-3 times until you have reproducible data.