Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

Which database could provide the signal sequence for targeting various cellular regions? ?

I am searching for thee database which could provide me signal sequence specific to the various cell organelle. such as signal sequence for mitochondria, signal sequence for endoplasmic...

04 February 2021 6,650 2 View

Is it possible for Cre positive mice to be negative for flox reporter gene (eGFP)?

I was genotyping some mice tail snips. It is a Cre Lox reporter mouse. The Cre is expressed using CRH promoter in the mouse brain and eGFP is the reporter gene. I isolated g-DNA and used it for...

12 January 2021 6,875 3 View

How can we increase awareness about Covid 19 prevention in our city among the local population?

The general public must be made aware of the mode of transmission, presenting symptoms and the measures that can be undertaken to prevent the spread of infection. Few options- Media, Webinars...

03 January 2021 3,538 13 View

How to remove rotation along z-axis and shift the origin to body's center?

I have attached the files with this post to explain me how to remove rotation and center the origin at the body's local coordinate. This is just one sample and I have 1000s of files like this. I...

18 December 2020 3,838 3 View

How can we know the exact soil taxonomy of any particular site. Is there any online available database for Indian soil taxonomy based on geography?

Please If one can let know the soil taxonomy for vindhyan region falling in Mirzapur district of Uttar Pradesh, India.

14 December 2020 9,879 14 View

Does anyone knows a tool (GOMS based or other) to automate calculation of task completion time?

Hello good human, really hope that you are doing well. I have been reading "The Psychology of Human Computer Interaction" by Stuart Card et al. They have introduced one of the ground breaking...

11 December 2020 7,269 3 View

Is there a solvent for polypropylene at room temperature?

I want to dissolve polypropylene for making a solution so as to make fibres at room temperature. I have tried toluene and xylene but without any success in dissolving.

06 December 2020 8,479 2 View

No title

30 November 2020 5,089 5 View

How to upload the name of notable journal The Business & Management Review (Print) when it is not showing in dropdown list?

I am also unable to upload notable journal The Business & Management Review (Print) with ISSN 2047-2854 (Print) ISSN 2047-2862 (ONLINE) Indexing with: ProQuest, Ebsco Host, EconBiz,...

24 June 2020 3,125 3 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

Can anyone recommend utilizing the plasmid extraction kit for extraction of the circular DNA genome of viruses associated with insect vectors?

Dear Colleagues, I am planning to detect and discover DNA viruses particularly those with a circular genome and transmitted by insect vectors. However, I am confused about selecting the best...

10 January 2021 9,534 4 View

Is BRSV ("bovine respiratory syncytial virus") less susceptible to RNA degradation than SARS-CoV-2 when thawing a sample?

I've noticed that when thawing long-frozen samples (3-4 months at -20 C), most of the SARS-CoV-2 virus does not remain intact, but that the surrogate appears to. Has anyone else noticed this?

09 January 2021 4,168 7 View

WURSS-11 - how to apply in research practice?

Hello, in the framework of my master's thesis, I would like to assess the efficacy of probiotic on upper respiratory tract infection (URTI) incidence and symptom ratings in athletes under...

07 January 2021 2,284 1 View

It does not appear logistically possible to vaccinate everyone for COVID. So what's the point?

Public health authorities claim that no one is safe from COVID-19 until every one is safe. This has translated into a goal to vaccinate everyone who can be vaccinated. Logistically, however, it...

06 January 2021 4,269 12 View

Any one can tell me primer sequences used for q PCR to quantify MERS-CoV related coronavirus?

MERS-CoV primers

31 December 2020 2,389 2 View

Has anybody knows about Anti-COVID-19 antibody- anti-Viral COVID-19 Nucleocapsid (NP) Humanized Coronavirus?

In order to establish new Rapid Diagnostic Test, I need the mentioned Anti-COVID-19 antibody urgently. Has anybody can assist me to prepare it? Thanks in anticipated

31 December 2020 635 5 View

Are the mice expected to develop obvious airway disease after HDM? Will they have signs of respiratory distress at any time during the study?

I am developing model of asthma in mice using HDM. I was asked this question in order to submit the a protocol for developing a disease in animals. Searching in the literature, I can not find any...

30 December 2020 6,455 3 View

Will the serum of healed patients can be considered to make a vaccine/drug against COVID-19?

Will the serum of healed patients can be considered to make a vaccine/drug against COVID-19?

22 December 2020 2,180 4 View