Does anyone know of an effective, selective antagonist against the DREADD hM4Di?

07 July 2017 0 2K Report

Given the slow washout of CNO, it would be great if one could stop its effects on hM4Di with some drug (that ideally does not affect native M4 receptors). Bryan Roth mentioned in his 2016 Neuron Primer "DREADDs for Neuroscientists" that

"creation of DREADD antagonists would also be potentially highly useful", so apparently at that time there still was none. I would think it might be possible to create/discover something that eg blocks the CNO binding site, but so far found nothing!

I only found a paper from 2008 that looked into this a bit but did not find any antagonists (https://www.ncbi.nlm.nih.gov/pubmed/18628403). If anyone knows of anything, would be great to hear about it!

Badges
Science topic

Similar topics
Clinical Pharmacology
Dosing

More John J Tukker's questions See All

Technically: Do proctoring software add to quality assurance in assessment in blended learning modules?

One of the challenges in ensuring quality and integrity in blended learning modules is cheating especially when it comes to assessment (Tests, exams, and assignments). Now one of the solutions...

21 July 2024 6,741 0 View

Did Einstein misunderstand the photo-electric effect?

Photo-electric effect A light particle moving towards a surface cannot (by collision) force an electron particle to move away from that surface. A light wave can instead make interference with a...

19 July 2024 8,389 9 View

Can we find the Gromacs version from XTC file or TRR?

Hello everyone, This soudns like a strange question, but I created five XTC files using five different versions of GROMACS some years ago. All other files are missing, and I want to repeat the...

16 July 2024 7,205 2 View

Two PhD and One Master to expand R&D team, anyone interested?

Hi all, if you are looking for PhD and Master opportunity to work on Microalgae research and Biosensor for bacteria detection. Please do not hesitate to email me [email protected]

07 July 2024 7,576 0 View

Is light quantisation an illusion?

The photo-electric effect does not support quanta in light, since a light particle moving towards a surface cannot cause an electron to move away from the same surface at a right angle. Instead, a...

06 July 2024 2,288 35 View

Is gravity an EMERGENT phenomenon?

Newton found the law of gravity - but did not understand it. Fatio understood the law - but he was not accepted. Fatio assumed fast particles moving in all directions. Stability in planetary...

30 June 2024 9,284 28 View

Any automatic way to outline an image to measure the area in pixels?

Hi, I am trying to measure the area of the caudal fin in zebrafish, to study regeneration. I need to measure the area of the regenerating fin every 3 days after injury. So far, i have been using...

27 June 2024 5,068 1 View

Unstructured Interview Design?

In my qualitative study, I am trying to avoid the traditional belief that types of qualitative research design are just grounded theory, ethnography, phenomenology, case study, historical, and...

15 June 2024 3,884 1 View

Considering the issues with the ERP of GCTU, management has asked that you advise them on cloud computing models.?

Considering the issues with the ERP of GCTU, management has asked that you advise them on cloud computing models. You are expected to give 3 advantages and disadvantages of each of the models....

09 June 2024 5,629 0 View

Can I or you add publications to the page of Adri Jacobs?

Dear ResearchGate, I came across the ResearchGate page of Adri Jacobs, who passed away in 2010. This page contains 25 publication of Adri mainly from the period 2000-2008. One paper (2011) must...

01 June 2024 8,763 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How to conduct a sensitivity power analysis for Kendall's Tau?

Is there a straightforward way to conduct a sensitivity power analysis for a Kendall's Tau correlation? I was considering using the sensitivity setting and "Correlation: point biserial model" test...

28 July 2024 6,133 8 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View