John J Tukker

4 Questions 9 Answers 0 Followers

Questions related from John J Tukker

Does anyone have experience with the Leica Perfusion One/Two system(s)?

I was looking around for a new perfusion pump for mouse transcardial perfusion and fixation of the brain (and subsequent IHC), and came across this Perfusion Two system from Leica which not only...

21 July 2017 8,280 3 View

Does anyone know of an effective, selective antagonist against the DREADD hM4Di?

Given the slow washout of CNO, it would be great if one could stop its effects on hM4Di with some drug (that ideally does not affect native M4 receptors). Bryan Roth mentioned in his 2016 Neuron...

07 July 2017 1,953 0 View

What would be a good behavioral task to test mice on navigational difficulties also experienced by Alzheimer's patients?

AD patients tend to wander and get lost/disoriented. I'm not sure if the standard Morris Water Maze is necessarily the best task to test AD mouse models on, in relation to this question. I'm...

16 November 2013 2,582 17 View

Has anyone tried/succeeded to do whole-cell patch-clamp recordings from aged mice (or rats) in vivo?

It's clear that this may be difficult, but considering that some people do patch in slices from aged rats I thought someone must have at least tried. The idea is to look at mouse models of AD and...

24 September 2013 8,842 19 View