Hi, Take milk sample (2 ml) in a test tube and add 2 ml of 90%H2SO4 containing traces FCl2 from the side of test tube slowly.formation pink or purple ring in top milk tube at the indirect of

formaldehyde is present.

Hello Faris... You may try these methods:

Leach Test Method : Take about 5 ml of milk in a test tube and to this add equal volume of Ferric Chloride reagent. Allow the contents to mix and then keep the tube in boiling water bath for about 3-4 min. and observe the color of the solution in the tube.

Appearance of brownish pink color confirms the presence of formalin in the sample, whereas; control sample will remain white

Ferric Chloride reagent Preparation: Weigh 10 g of ferric chloride and dissolve it in distilled water to obtain 100 ml 10% ferric chloride solution.Take 1 ml of this above solution in a 500 ml volumetric flask and make up the volume using concentrated hydrochloric acid.

Chromotropic Acid Test: Take 1 ml of milk sample in a test tube and add 1 ml of the Chromotropic acid reagent and mix well. Appearance of yellow color confirms the presence of formalin in the sample, whereas; control sample will remain white (translucent).

Chromotropic acid reagent: Saturated solution of 1,8 dihydroxynaphthalene-3, 6-disulphonic acid in about 72% sulfuric acid (about 500 mg/100 ml). Light straw-colored solution should result.

Thanks for all

I need a quantitative determination of formaldehyde in milk.

Best reguards

I interest .....

Dear Esteemed Colleague,

Greetings. I trust this message finds you well and thriving in your scientific inquiries, particularly in the crucial field of food safety and quality control. Your query regarding a simple method for the determination of formaldehyde in milk and milk products is both timely and significant, given the importance of ensuring the safety of dairy consumption. Below, I outline a structured approach to address this analytical need, highlighting titration as a primary method along with an alternative technique for your consideration.

Titrimetric Method for Formaldehyde Determination

The titrimetric method, specifically the acetylacetone method, is a well-established procedure for quantifying formaldehyde in various matrices, including dairy products. This method is based on the reaction of formaldehyde with acetylacetone in the presence of ammonia or ammonium ion, forming a yellow dihydrolutidine derivative that can be quantitatively measured.

Procedure Overview:

Alternative Method: Spectrophotometric Analysis

For those seeking an alternative to titration, spectrophotometric analysis offers a viable option, leveraging the same chemical reaction between formaldehyde and acetylacetone.

Procedure Overview:

Considerations and Best Practices

• Interferences: Both methods require attention to potential interferences in the milk matrix. Pre-treatment steps, such as protein precipitation or fat removal, may enhance accuracy.
• Quality Control: Implement quality control measures, including the use of blanks and formaldehyde standards, to ensure the reliability of results.
• Safety: Handle all chemicals, especially formaldehyde and acetylacetone, with appropriate safety precautions to protect the analyst and the environment.

By adopting these methodologies, you can achieve reliable quantification of formaldehyde in milk and milk products, contributing to food safety and consumer protection. Should you require further details or have specific questions about the procedures, please do not hesitate to reach out. I am here to assist in advancing your research and analytical capabilities.

Warm regards.

With this protocol list, we might find more ways to solve this problem.