Anoxic protein purification - Impact of sonication on Fe-S cluster proteins?

Dear all, I need to purify anoxically a protein with Fe-S clusters (sensitive to oxygen). I used to do cell lysis using a French press but because the instrument is too big to be place in my...

08 November 2020 9,528 3 View

How to compute the effects in half fractions?

Greetings, I'm doing a screening design and want to allow for lack of fit. I need replications so I want to add four additional points to my dataset. Rather than use center runs I use corner...

11 October 2020 9,672 1 View

How to choose an ID variable in the HLM software student version ?

When I have to choose my identification variable in lvl 1 and lvl 2, I cannot choose it because it is grayed out. Do you know why ? and how to fix it?

27 March 2020 8,558 2 View

Meta-analysis in Stata : how to perform it with effect sizes ?

19 December 2019 9,553 2 View

How to find 3 initial parameters for gompertz curve in presence of other predictors

Given the following when fitting a three parameter gompertz curve to a predictor xg(x) = alpha * exp(- gamma * exp(- beta *x) ) I need to know whether gamma is equal to the inflection point of...

13 May 2017 2,803 11 View

How to find initial parms estimates when doing nonlinear analysis?

proc NLIN data = eleven BEST = 10 MAXITER = 100 METHOD = Gauss CONVERGE=1.0E-6 LIST ALPHA = 0.05 OUTEST = outest(where = (_TYPE_ = "COVB")); parms B1 = 1 B2 = .5128 B3=-.0036; model y =...

26 March 2017 2,174 5 View

Does anyone know how to extract with Matlab the georeferencing information of a JP2 image?

I'd like to read with Matlab the georeferencing metadata contained in a JP2 image. Any idea ? Thanks !

18 June 2015 859 4 View

Separation of 100 and 150 kD proteins in Westerns?

I'm trying to dramatically separate 150kd proteins from 100kd proteins in an acrylamide gel - more than what a 4% resolving gel would accomplish. Any ideas? Is this even possible? Thanks!

18 February 2015 5,157 7 View

What is the best housekeeping gene for a Western blot on purified adipocytes? GAPDH disappears after the purification.

Western blot

19 December 2012 6,810 5 View

Is it appropriate to use AIDA model (Assessment of Identity Development in Adolescence) on a non-clinical sample?

I am researching emotion regulation and identity development in adolescence. I've come across several inventories for assessing identity development and it seems like the AIDA scale is the most...

16 February 2021 1,261 1 View

Prompt research in the direction of "deprivation and adolescents" please?

01 February 2021 6,401 3 View

Who to create single SPSS data sheet for different measures by two different populations??

I am working on Effect of parents personality patterns on adolescents, I have collected data from parents and adolescents by using different scales on both population. Now I have to create SPSS...

26 December 2020 7,238 3 View

Recommendations for adolescent Stressful (Traumatic) Life Events Checklist?

Greetings! I am working on a research project that includes adolescent stressful life events as one of the variables. As an instrument to measure this construct, I found the Life Events Checklist...

20 October 2020 7,890 5 View

Is there any tool for Screening Specific Learning Disability for Class or grade 9 and above? Please Suggest?

Doing research in the area of Psychosocial implications for Specific Learning Disability with adolescents. So need a tool to screen out children and adolescents from class 9 and above for LD.

12 September 2020 1,573 4 View

I need the adolescent (12 to 18 years old) brain T1 and diffusion weighted images data set. where can I find them?

I am a university student and I am doing research in medical image processing. I need the adolescent (12 to 18 years old) brain T1 and diffusion-weighted images data set. Actually , I really need...

09 September 2020 4,805 3 View

Online self reported survey on nutrition and physical activities of children aged 11-12 years old?

Do you have some recommendation for this this type of survey? Some standard form of survey? I am looking for some short form, that can be easily done by children.

25 July 2020 2,641 3 View

Recommended cutpoints for hip-worn actigraph measuring physical activity in pregnant teens?

From the literature we've seen research mostly examines pregnancy in adults (>20 years), but our population is 14-18 years old. We'd love any feedback on if we should go with cutpoints for...

09 June 2020 2,981 1 View

Is there a questionnaire about the psychological effects of COVID-19 on adolescents and young people?

Dear researchers, I want to do some research about the psychological effects of COVID-19 on adolescents and young people. To do this, I need a standard questionnaire.

13 April 2020 7,254 11 View

How to prevent non-normative behaviors developed from the consumption of online pornography by adolescents and young people?

Our investigation group is currently working on a research about pornography consumption in adolescents and young people. We'd like to read your comments and any information you consider...

22 March 2020 6,127 3 View