I have a perfusion well-chamber with hole on it. I want to seal the hole with parafilm
Do you think disinfection with 70% ethanol and UV light would be enough?
through the hole parafilm will have direct contact with media
I have sterilized cover glasses using only UV and without having contamination problems later.
that is my experience
be lucky!
sorry my english!
You can probably add in H2O2 disinfection BEFORE the EtOH. Not sure it will be enough but do not see many options, unless you have a gamma radiation facility...
Parafilm is ideal for the scientific laboratory because it is a thermoplastic, self-sealing film. It holds moisture loss to a minimum. It offers excellent protection for the contents of tubes, flasks, and petri dishes. The unique properties of the film enable it to cling around even irregular shapes and convoluted surfaces. Parafilm M can be stretched 3-4 times its original length before breaking.
Parafilm M makes the perfectly hydrophobic surface for transferring small amounts of reagent to a TEM grid. Dispense a single drop of reagent onto the Parafilm surface, it will form a nice non-spreading "bead" against which a grid can be "touched" to the liquid, picking up a controlled amount of liquid by the grid. Especially useful where expensive reagents are involved. Can also be used for picking up floating grids and films firm liquid surfaces.
Parafilm M makes a superior bandage cover in drainage cases. It is useful in all hospital laboratories and medical stocking areas. It is ideal when used as a tray and shelf liner to prevent slippage and breakage of bottles, containers, and instruments.
Parafilm M can be sterilized using either radiation techniques or hydrogen peroxide. Parafilm is not considered a sterile product in its as received state.
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