Does anyone know how to reproduction of Vaccinium artostaphylos L. (worthberry) with tissue culture method?
What is the best media fore reproduction of worthberry?
Micropropagation of Vaccinium arctostaphylos L. (Ericaceae) via lateral-bud cultures . Stems are more likely to form reproductive terminal buds or lateral shoot buds are used as explant sources. Anderson’s rhododendron medium (AN), McCown’s woody plant medium (WPM), and Murashige and Skoog
(MS) basal medium, each supplemented with zeatin/indole-3-butyric acid (IBA) (1.0/0.1 mg L–1), are used as best basal medium for multiple shoot formation; WPM is found the most effective basal medium for this purpose. Subsequent shoot multiplication and development studies is carried out with WPM supplemented with an auxin, IBA (0.1 mg L–1), and 2 different cytokinins, zeatin and thidiazuron (TDZ), in various concentrations (0.5, 1.0, and 2.0 mg L–1,respectively). After a 6-week photoperiod (16/8 h, light/ dark) application, zeatin/IBA combinations are found to be the most suitable for shoot multiplication and growth. For achieving highest shoot length medium supplemented with 1.0/0.1 mg L–1 zeatin/IBA, while the highest increases in leaf number and multiple shoot proliferation can be obtained from medium supplemented with 2.0/0.1 mg L–1 zeatin/ IBA. WPM is also employed as a rooting medium supplemented with different concentrations of IBA (from 0.5 mg L–1 to 8.0 mg L–1) and a 16/8 photoperiod or a completely dark regime. At this stage, medium is supplemented with 0.5 mg L–1 IBA for best rooting.
Thank you very much Dear Ravi Kant. I appreciated your valuable explanation.
Best wishes
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