Does anyone know how to quantify inflammatory cells using ImageJ?

More Haley Anne Hopper's questions See All

Is M9 salts media compatible with HPLC?

I am culturing E. coli in 1X M9 salt media with plastic fragments and want to perform HPLC for analysis of degradation products. The plastic fragments will be removed prior to analysis, but do I...

28 November 2023 8,400 4 View

What are the major differences between E. coli HB101 and BL21?

I am trying to choose a strain for expression of a plasmid. Which one would be better?

06 September 2023 6,603 1 View

Can you please identify this centric diatom?

This is a centric species with coarse areolae and two thick processes on opposite sides of the valve. Is it a kind of Aulacoseira? or Actinocyclus? Doesn't seem to fit the definitions for either...

14 August 2023 6,539 4 View

Where can I purchase Ideonella sakaienses from?

Looking for a positive control for a microplastic degradation study and would like to use Ideonella sakaienses but I cannot find anywhere to purchase it.

07 March 2023 8,123 0 View

How to design PCR primers using SnapGene?

I am trying to design primers to target either TagBFP or mTagBFP2 protein regions of a plasmid. However, these regions are 693 and 711 base pairs long, respectively. I understand the guidelines...

25 January 2023 3,295 5 View

What are some new-age techniques that are frequently overused?

Current research often uses new, next-generation, "flashy" experimental techniques (i.e. single-cell RNA-seq) that have replaced some of the older, smaller, yet fundamental experimental...

12 January 2023 2,792 4 View

Visualizing Protein Interactions in PyMOL?

I am trying to visualize the protein interactions between human lung surfactant protein D (1PW9) and the SARS-CoV-2 spike (6VYB). I used PatchDock and FireDock to generate the structure and put...

14 July 2022 5,183 2 View

How should I treat A549 cells with Pseudomonas LPS?

I am trying to treat A549 cells with Pseudomonas LPS to stimulate antimicrobial peptide expression. How should I go about this? I am not sure where to start or what to do.

05 July 2022 2,308 1 View

Is there a reagent I can resuspend a frozen cell pellet in that would allow me isolate RNA and Protein from the same pellet?

Hi researchers, I was wondering if anyone knows a way I could Isolate RNA and Protein from the same cell pellet. I'm not looking for a kit that allows me to do both at the same time unless that...

22 March 2022 8,965 7 View

Does anyone know of a good paper that includes the GRADE checklist for measuring study quality?

Hello, I am looking for a GRADE checklist for measuring study quality in my systematic review and meta-analysis study. Does anyone have any recommendations of papers that include this?

25 January 2022 2,501 3 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Aboma Merdasa

If this question is simply image processing I can be of assistance. If this question regards more than just a technique of counting objects in an image, I might not be as much of assistance. If you are interested, you can write me and I can send you some code.

Deirdre Edge

Hi Xavi Langa Oliva, if possible could you share your answer?

Hisham Abada

Could anyone share the answers please.