I am trying to treat A549 cells with Pseudomonas LPS to stimulate antimicrobial peptide expression. How should I go about this? I am not sure where to start or what to do.
Just extract LPS from your Pseudomonas, dilute it and treat your cells.
I am culturing E. coli in 1X M9 salt media with plastic fragments and want to perform HPLC for analysis of degradation products. The plastic fragments will be removed prior to analysis, but do I...
28 November 2023 8,400 4 View
I am trying to choose a strain for expression of a plasmid. Which one would be better?
06 September 2023 6,603 1 View
This is a centric species with coarse areolae and two thick processes on opposite sides of the valve. Is it a kind of Aulacoseira? or Actinocyclus? Doesn't seem to fit the definitions for either...
14 August 2023 6,539 4 View
Looking for a positive control for a microplastic degradation study and would like to use Ideonella sakaienses but I cannot find anywhere to purchase it.
07 March 2023 8,123 0 View
I am trying to design primers to target either TagBFP or mTagBFP2 protein regions of a plasmid. However, these regions are 693 and 711 base pairs long, respectively. I understand the guidelines...
25 January 2023 3,295 5 View
Current research often uses new, next-generation, "flashy" experimental techniques (i.e. single-cell RNA-seq) that have replaced some of the older, smaller, yet fundamental experimental...
12 January 2023 2,792 4 View
I am trying to visualize the protein interactions between human lung surfactant protein D (1PW9) and the SARS-CoV-2 spike (6VYB). I used PatchDock and FireDock to generate the structure and put...
14 July 2022 5,183 2 View
Hi researchers, I was wondering if anyone knows a way I could Isolate RNA and Protein from the same cell pellet. I'm not looking for a kit that allows me to do both at the same time unless that...
22 March 2022 8,965 7 View
Hello, I am looking for a GRADE checklist for measuring study quality in my systematic review and meta-analysis study. Does anyone have any recommendations of papers that include this?
25 January 2022 2,501 3 View
I am trying to run a western blot on A549 cells with beta-actin as the loading control and alpha-SMA as the protein of interest. However, both beta-actin and alpha-SMA have similar kDas. How would...
04 December 2021 7,527 3 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View