Does anyone know how to get/buy the competent strain of S. aureus RN4220? My research group is interested in study the role of mutations in Vra and Gra genes obtained from hVISA strains from Brazil. For that we need a competent S. aureus strain and has been very difficult to get it. Thanks.
Thanks a lot dear Sudip!
Thanks all for attention. The strain is not available at ATCC.
I doubt this strain is available commercially. I believe it was generated over 30 years ago in Richard Novick's lab (hence the RN) and is probably found in most labs around the world studying staphylococci that perform genetic manipulations. You could search for a local lab working on staphs that may have this strain or request it directly from Novick. Most labs are willing to, and indeed should, share published strains.
http://www.med.nyu.edu/microbiology-parasitology/faculty/novick-lab-microbiology
Have you taken a look at the Network on Antibiotic Resistance in Staph. Aureus? (narsa.net), now bei resources. They have those strains available.
Staphylococcus aureus subsp. aureus RN4220
Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies.
you consult
NCBI
I may be incorrect here but an internet search showed that S. aureus RN4220 is also known as S.aureus ATCC 35556. I notice that you have mentioned that this organism is not available at ATCC. I have however found that S.aureus ATCC 35556 and is available on the website - hoping this is correct :)
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