I want to identify some proteins in serum samples from human patients. I have reviewed literature and is better to deplete high-abundance proteins such as albumin and transferrin. I have read that DTT is a good methodology, although I am not sure if the last step is necessary. So, you add DTT to serum samples, incubate for 60 min, centrifuge and, does the supernatant has to be evaporated and re-suspended or should I use it directly for western blotting experiments?

Thank you very much!!