What are the limitations for colorimetric in computer vision technique to extract object features with noisy background?

Combination of morphometric and colorimetric has been applied for feature extraction in detection algorithm. In noisy background, this CV technique facing wrong identification results as well as...

07 August 2018 7,661 7 View

What is the relationship between convolution of high frequencies image with a 3 x 3 Laplacian kernel?

Filtering dataset quality is very important prior to segmentation analysis. A single channel of an image is applied before convolve it with the Laplacian kernel.

31 December 2017 9,824 0 View

What is the difference between illumination normalization techniques, ANN classifier and CNN classifier in solving face recognition problems?

This is specific to the face recognition in unconstrained environment, generalization capability of the face recognition systems with large training datasets and generation of additional synthetic...

11 December 2017 960 2 View

What is the meaning of strel, 3 x 3 matrix of all ones?

The same strel is apply for dilation and erosion operation in morphological operator.

11 December 2017 494 0 View

What is the definition and function of multiresolution segmentation in image analysis?

It was reported as a basic procedure in the software eCognition for objectoriented image analysis (Delphi2 Creative Technologies, Munich).

03 April 2017 3,254 0 View

How image intensity can be changed using the logical indexing method?

This subject is discussed under indexing in MATLAB. Basic script, Img (Img=0) = 1 (All zero intensities changed to one), or can use Img>200, depending on the aforementioned tech. used (one can...

03 April 2017 4,631 4 View

In MATLAB function, how to generate images with correlated pixels?

It is more realistic to model pixels in an images with some degree or type of correlation, because due to the point spread function and the intensity of a pixel may contain info from its...

03 April 2017 2,158 0 View

What is the relation between "Bremsstrahlung" signal and plasmons signal?

If a part of the energy that an electron carries is transferred to the specimen, several processes might occur leading to the generation of the above signals.

31 December 2016 5,573 0 View

How to determine sample size for any spectroscopic experiment?

Is there any formula or specific program?

11 December 2016 530 6 View

What is the detector for NMR spectroscopy and SEM/TEM?

To understand basic of various spectroscopy techniques, type of detector of electron with characteristic kinetic energy is important to be explained.

11 December 2016 319 0 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

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22 February 2021 9,932 11 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View