Does anyone know how many runs can be done on a single tank of buffer before replacing it?

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What are the limitations for colorimetric in computer vision technique to extract object features with noisy background?

Combination of morphometric and colorimetric has been applied for feature extraction in detection algorithm. In noisy background, this CV technique facing wrong identification results as well as...

07 August 2018 7,773 7 View

What is the relationship between convolution of high frequencies image with a 3 x 3 Laplacian kernel?

Filtering dataset quality is very important prior to segmentation analysis. A single channel of an image is applied before convolve it with the Laplacian kernel.

31 December 2017 9,956 0 View

What is the difference between illumination normalization techniques, ANN classifier and CNN classifier in solving face recognition problems?

This is specific to the face recognition in unconstrained environment, generalization capability of the face recognition systems with large training datasets and generation of additional synthetic...

11 December 2017 1,090 2 View

What is the meaning of strel, 3 x 3 matrix of all ones?

The same strel is apply for dilation and erosion operation in morphological operator.

11 December 2017 661 0 View

How image intensity can be changed using the logical indexing method?

This subject is discussed under indexing in MATLAB. Basic script, Img (Img=0) = 1 (All zero intensities changed to one), or can use Img>200, depending on the aforementioned tech. used (one can...

03 April 2017 4,793 4 View

What is the definition and function of multiresolution segmentation in image analysis?

It was reported as a basic procedure in the software eCognition for objectoriented image analysis (Delphi2 Creative Technologies, Munich).

03 April 2017 3,385 0 View

In MATLAB function, how to generate images with correlated pixels?

It is more realistic to model pixels in an images with some degree or type of correlation, because due to the point spread function and the intensity of a pixel may contain info from its...

03 April 2017 2,298 0 View

What is the relation between "Bremsstrahlung" signal and plasmons signal?

If a part of the energy that an electron carries is transferred to the specimen, several processes might occur leading to the generation of the above signals.

31 December 2016 5,725 0 View

What is the detector for NMR spectroscopy and SEM/TEM?

To understand basic of various spectroscopy techniques, type of detector of electron with characteristic kinetic energy is important to be explained.

11 December 2016 447 0 View

How to determine sample size for any spectroscopic experiment?

Is there any formula or specific program?

11 December 2016 642 6 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

What is the best method for removing paraffin from plant samples prepared for microtome?

...

24 July 2024 3,087 3 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View

How can EDTA be removed from already extracted DNA samples?

Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...

13 July 2024 1,675 3 View

Jazmín Aguilar Medina

Agarose electrophoresis?, I use it at least for 5 runs, works well but I have to added few ml than are losing by evaporation

Mohd Najib Ahmad

For DGGE electrophoresis. How about buffer at 0.5x TAE?

The recommendation is use it only once, but i know than in a near laboratory it is used for twice with buffer at 1x TAE without problems